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Determination of residual cell culture media components by MEKC
Journal article   Peer reviewed

Determination of residual cell culture media components by MEKC

Junge Zhang, Utpal Chakraborty and Joe P Foley
Electrophoresis, v 30(22), pp 3971-3977
Nov 2009
DOI: https://doi.org/10.1002/elps.200900169
PMID: 19876960
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Niacin - analysis Xanthine - analysis Chromatography, Micellar Electrokinetic Capillary - methods Limit of Detection Reproducibility of Results Mycophenolic Acid - analysis Riboflavin - analysis Hypoxanthine - analysis Folic Acid - analysis Sensitivity and Specificity Sodium Dodecyl Sulfate - pharmacology Culture Media - chemistry Hydrogen-Ion Concentration
Folic acid, hypoxanthine, mycophenolic acid, nicotinic acid, riboflavin, and xanthine are widely used as cell culture media components in monoclonal antibody manufacturing. These components are subsequently removed during the downstream purification processes. This article describes a single MEKC method that can simultaneously determine all the listed compounds with acceptable LOD and LOQ. All the analytes were successfully separated by MEKC using running buffer containing 40 mM SDS, 20 mM sodium phosphate, and 20 mM sodium borate at pH 9.0. The MEKC method was compared to the corresponding CZE method using the same running buffer containing no SDS. The effect of SDS concentration on separation, the pH of the running buffer, and the detection wavelength were studied and optimal MEKC conditions were established. Good linearity was obtained with correlation coefficients of more than 0.99 for all analytes. Specificity, accuracy, and precision were also evaluated. The recovery was in the range of 89-112%. The precision results were in the range of 1.7-4.8%. The experimentally determined data demonstrated that the MEKC method is applicable to the determination of the six analytes in in-process samples from monoclonal antibody manufacturing processes.

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9 citations in Web of Science
8 citations in Scopus

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Collaboration types
Industry collaboration
Domestic collaboration
Web of Science research areas
Biochemical Research Methods
Chemistry, Analytical
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