Journal article
Determination of the membrane topology of Arv1 and the requirement of the ER luminal region for Arv1 function in Saccharomyces cerevisiae
FEMS yeast research, v 11(6), pp 524-527
Sep 2011
PMID: 21539707
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In Saccharomyces cerevisiae, ARV1 encodes a 321 amino acid transmembrane protein localized to the endoplasmic reticulum (ER) and Golgi. It has been shown previously that arv1 cells harbor defects in sphingolipid and glycosylphosphatidylinositol biosyntheses, and may harbor sterol trafficking defects. Using C-terminal fusion to Suc2-His4, we determined the orientation of full-length Arv1 in the ER membrane. Once membrane topology was determined, we used this information and truncation analysis to establish the minimum protein length required for Arv1 function and phenotypic suppression. By understanding the topology of Arv1 we can now further analyze its putative lipid and glycosylphosphatidylinositol intermediate transport activities.
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Details
- Title
- Determination of the membrane topology of Arv1 and the requirement of the ER luminal region for Arv1 function in Saccharomyces cerevisiae
- Creators
- Michelle L. Villasmil - Target (United States)Joseph T. Nickels - Target (United States)
- Publication Details
- FEMS yeast research, v 11(6), pp 524-527
- Publisher
- Oxford Univ Press
- Number of pages
- 4
- Grant note
- Medical Diagnostic Laboratories, L.L.C.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000293684200009
- Scopus ID
- 2-s2.0-79961208155
- Other Identifier
- 991021229997604721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology
- Mycology