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Journal article
Determining Whether Agonist Density or Agonist Number Is More Important for Immune Activation via Micoparticle Based Assay
Frontiers in immunology, v 11, 642
Apr 2020
PMID: 32328073
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
It is unknown if surface bound toll-like-receptor (TLR) agonists activate cells via density or total molecular number. To answer this question, we developed a TLR agonist surface conjugated polystyrene microparticle (MP) system. Using a library of MPs with varying TLR agonist density and number, we simultaneously observed innate immune cell MP uptake and TNFα expression using ImageStream flow cytometry on a cell by cell basis. The data shows that total TLR number and not density drives cellular activation with a threshold of approximately 10
-10
TLR agonists. We believe that this information will be crucial for the design of particulate vaccine formulations.
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Details
- Title
- Determining Whether Agonist Density or Agonist Number Is More Important for Immune Activation via Micoparticle Based Assay
- Creators
- Peter Deak - Drexel University, Chemical and Biological EngineeringFlora Kimani - University of ChicagoBrittney Cassaidy - University of ChicagoAaron Esser-Kahn (Corresponding Author) - University of Chicago
- Publication Details
- Frontiers in immunology, v 11, 642
- Publisher
- Frontiers
- Number of pages
- 8
- Grant note
- U01 AI124286 / NIAID NIH HHS F32 AI147517 / NIAID NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000529925200001
- Scopus ID
- 2-s2.0-85083879544
- Other Identifier
- 991019376908604721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Immunology