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Journal article
Development and Validation of a Sensitive LC-MS/MS Method for the Determination of D-Serine in Human Plasma
Journal of pharmaceutical and biomedical analysis, v 89, pp 1-5
30 Oct 2013
PMID: 24247087
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A validated LC-MS/MS method was developed for the determination of D-Serine in human plasma. The method was fully validated for use with human plasma samples and was linear from 0.19 nmol/ml to 25 nmol/ml. The coefficient of variation was ≤ 5% for the high QC standards and ≤ 8% for the low QC standards in plasma. D-Serine and L-Serine were resolved by pre-column derivatization using (R)-1-Boc-2-piperidine carbonyl chloride as the derivatizating agent. The method was used to determine the concentration of D-Serine in plasma samples obtained in patients receiving a continuous 5-day intravenous infusion of (R,S)-ketamine. The changes in D-Ser levels varied in the 6 patients, with circulating D-Ser levels increasing as much as 35% in a patient, while decreasing 20% in a patient. While only preliminary data, the results suggests the potential importance in determining the D-Ser levels in plasma and their potential role in physiological response.
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Details
- Title
- Development and Validation of a Sensitive LC-MS/MS Method for the Determination of D-Serine in Human Plasma
- Creators
- Y. Xie - Shanghai Jiao Tong UniversityG.M. Alexander - Drexel UniversityR.J. Schwartzman - Drexel UniversityN. Singh - Laboratory of Clinical Investigation, Division of Intramural Research Programs, National institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.M. Torjman - Cooper Medical School of Rowan UniversityM. Goldberg - Cooper Medical School of Rowan UniversityI.W. Wainer - Cooper Medical School of Rowan UniversityR. Moaddel - Laboratory of Clinical Investigation, Division of Intramural Research Programs, National institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
- Publication Details
- Journal of pharmaceutical and biomedical analysis, v 89, pp 1-5
- Publisher
- Elsevier
- Grant note
- Z99 AG999999 || AG / National Institute on Aging : NIA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]; Neurology
- Web of Science ID
- WOS:000330487000001
- Scopus ID
- 2-s2.0-84887911758
- Other Identifier
- 991019168423204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Analytical
- Pharmacology & Pharmacy