Journal article
Development and characterization of positively selected brain-adapted SIV
Virology journal, Vol.2(1), pp.44-44
12 May 2005
PMCID: PMC1145188
PMID: 15890081
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
HIV is found in the brains of most infected individuals but only 30% develop neurological disease. Both viral and host factors are thought to contribute to the motor and cognitive disorders resulting from HIV infection. Here, using the SIV/rhesus monkey system, we characterize the salient characteristics of the virus from the brain of animals with neuropathological disorders. Nine unique molecular clones of SIV were derived from virus released by microglia cultured from the brains of two macaques with SIV encephalitis. Sequence analysis revealed a remarkably high level of similarity between their
env
and
nef
genes as well as their 3' LTR. As this genotype was found in the brains of two separate animals, and it encoded a set of distinct amino acid changes from the infecting virus, it demonstrates the convergent evolution of the virus to a unique brain-adapted genotype. This genotype was distinct from other macrophage-tropic and neurovirulent strains of SIV. Functional characterization of virus derived from representative clones showed a robust
in vitro
infection of 174xCEM cells, primary macrophages and primary microglia. The infectious phenotype of this virus is distinct from that shown by other strains of SIV, potentially reflecting the method by which the virus successfully infiltrates and infects the CNS. Positive
in vivo
selection of a brain-adapted strain of SIV resulted in a near-homogeneous strain of virus with distinct properties that may give clues to the viral basis of neuroAIDS.
Metrics
1 Record Views
Details
- Title
- Development and characterization of positively selected brain-adapted SIV
- Creators
- Peter J Gaskill - Scripps Research InstituteDebbie D Watry - Scripps Research InstituteTricia H Burdo - Scripps Research InstituteHoward S Fox - Scripps Research Institute
- Publication Details
- Virology journal, Vol.2(1), pp.44-44
- Publisher
- BioMed Central
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Drexel University; College of Medicine; Pharmacology and Physiology
- Identifiers
- 991020100073004721
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Web of Science research areas
- Virology