Journal article
Development and characterization of twenty-five new polymorphic microsatellite markers in proso millet (Panicum miliaceum L.)
Genes & genomics, v 32(3), pp 267-273
01 Jun 2010
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Abstract
Millets such as proso millet have excellent nutritional properties and could become a basic resource for crop breeding programs and food diversification. In this study, 25 polymorphic microsatellite markers were developed and characterized through construction of an SSR-enriched library from genomic DNA of proso millet (Panicum miliaceum L.). In total, 110 alleles were detected, with an average of 4.4 alleles per locus. Values of major allele frequency (M-AF) and expected heterozygosity (H-E) ranged from 0.36 to 0.98 (mean = 0.73) and from 0.04 to 0.74 (mean = 0.37), respectively. The mean genetic similarity coefficient was 0.3711, indicating that among 50 accessions of proso millet there was wide genetic variation. The newly developed microsatellite markers should be useful tools for assessing genetic diversity, understanding population structure, and breeding of proso millet.
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Details
- Title
- Development and characterization of twenty-five new polymorphic microsatellite markers in proso millet (Panicum miliaceum L.)
- Creators
- Young-Il Cho - Plant (United States)Jong-Wook Chung - Plant (United States)Gi-An Lee - RDA (United States)Kyung-Ho Ma - RDA (United States)Anupam Dixit - SVBP Univ Agr & Technol, Basmati Export Dev Fdn, Rice DNA & Qual Testing Lab, Meerut 250110, Uttar Pradesh, IndiaJae-Gyun Gwag - RDA (United States)Yong-Jin Park - Plant (United States)
- Publication Details
- Genes & genomics, v 32(3), pp 267-273
- Publisher
- Springer Nature
- Number of pages
- 7
- Grant note
- 20080401034058 / Rural Development Administration (RDA) of the Republic of Korea; Rural Development Administration (RDA), Republic of Korea
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000281669100010
- Scopus ID
- 2-s2.0-77957084890
- Other Identifier
- 991020547441604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biotechnology & Applied Microbiology
- Genetics & Heredity