Journal article
Development of SSR markers to study diversity in the genus Cymbidium
Biochemical systematics and ecology, v 38(4), pp 585-594
01 Aug 2010
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Abstract
Cymbidium spp. are important potted flowers with extremely high ornamental and economic value. The present study reports the development of 14 new simple sequence repeat (SSR) markers through the construction of an enriched
Cymbidium goeringii library and cross-amplification in
Cymbidium sinensis and
Cymbidium hybridium. Of 525, 322 (61.33%) clones had SSR motifs and among motifs di-nucleotides were predominant and followed by tri-nucleotide and tetra-nucleotide type. In polymorphic analysis using 14 newly developed SSRs, a total of 201 alleles across 96
Cymbidium accessions were detected with an average of 14.4 per locus. The average heterozygosity was 0.394. The average gene diversity and polymorphism information content values were 0.394 and 0.639, respectively. The mean genetic similarity coefficient was 0.4297, indicating a wide genetic variation among the
Cymbidium accessions. These newly developed SSRs will be useful tools for genotype identification, germplasm conservation, molecular breeding, and assessments of genetic diversity and population structure in
Cymbidium.
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Details
- Title
- Development of SSR markers to study diversity in the genus Cymbidium
- Creators
- Kyaw Thu Moe - Kongju National UniversityWeiguo Zhao - Kongju National UniversityHong-Seon Song - Kongju National UniversityYou-Hyen Kim - Kongju National UniversityJong-Wook Chung - Kongju National UniversityYoung-Il Cho - Kongju National UniversityPue Hee Park - RDA (United States)Ha-Seung Park - Yesan Chrysanthemum Experiment Station, Chungnam Provincial ARES, Yesan 340-915, Republic of KoreaSoo-Cheon Chae - Kongju National UniversityYong-Jin Park - Kongju National University
- Publication Details
- Biochemical systematics and ecology, v 38(4), pp 585-594
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000284565800016
- Scopus ID
- 2-s2.0-77958461427
- Other Identifier
- 991020547317104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Ecology
- Evolutionary Biology