Journal article
Development of a microfluidic device for detection of pathogens in oral samples using upconverting phosphor technology (UPT)
Annals of the New York Academy of Sciences, v 1098(1), pp 375-388
Mar 2007
PMID: 17435143
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Confirmatory detection of diseases, such as HIV and HIV-associated pathogens in a rapid point-of-care (POC) diagnostic remains a goal for disease control, prevention, and therapy. If a sample could be analyzed onsite with a verified result, the individual could be counseled immediately and appropriate therapy initiated. Our group is focused on developing a microfluidic "lab-on-a-chip" that will simultaneously identify antigens, antibodies, RNA, and DNA using a single oral sample. The approach has been to design individual modules for each assay that uses similar components (e.g., valves, heaters, metering chambers, mixers) installed on a polycarbonate base with a common reporter system. Assay miniaturization reduces the overall analysis time, increases accuracy by simultaneously identifying multiple targets, and enhances detector sensitivity by upconverting phosphor technology (UPT). Our microfluidic approach employs four interrelated components: (1) sample acquisition-OraSure UPlink collectors that pick-up and release bacteria, soluble analytes, and viruses from an oral sample; (2) microfluidic processing-movement of microliter volumes of analyte, target analyte extraction and amplification; (3) detection of analytes using UPT particles in a lateral flow system; and (4) software for processing the results. Ultimately, the oral-based microscale diagnostic system will detect viruses and bacteria, associated pathogen antigens and nucleic acids, and antibodies to these pathogens.
Metrics
Details
- Title
- Development of a microfluidic device for detection of pathogens in oral samples using upconverting phosphor technology (UPT)
- Creators
- William R Abrams - Department of Basic Sciences, New York University College of Dentistry, New York, New York 10010, USA. william.abrams@NYU.eduCheryl A BarberKurt McCannGary TongZongyuan ChenMichael G MaukJing WangAlex VolkovPete BourdellePaul L A M CorstjensMichel ZuiderwijkKeith KardosShang LiHans J TankeR Sam NiedbalaDaniel MalamudHaim Bau
- Publication Details
- Annals of the New York Academy of Sciences, v 1098(1), pp 375-388
- Publisher
- United States
- Grant note
- UO1DE0114964 / NIDCR NIH HHS UO1DE017855 / NIDCR NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Technology
- Web of Science ID
- WOS:000246091500026
- Scopus ID
- 2-s2.0-34248174353
- Other Identifier
- 991014877755704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Dentistry, Oral Surgery & Medicine
- Medical Laboratory Technology