Journal article
Development of a surface plasmon resonance (SPR) assay for rapid detection of Aeromonas hydrophila
Analytical biochemistry, v 670, 115151
01 Jun 2023
PMID: 37028781
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Aquaculture plays an increasingly important if not critical role in the current and future world food supply. Aeromonas hydrophila, a heterotrophic, Gram-negative, bacterium found in fresh or brackish water in warm climates poses a serious threat to the aquaculture industry in many areas, causing significant economic losses. Rapid, portable detection methods of A. hydrophila are needed for its effective control and mitigation. We have developed a surface plasmon resonance (SPR) technique to detect PCR (polymerase chain reaction) products that can replace agarose gel electrophoresis, or otherwise provide an alternative to costlier and more complicated real-time, fluorescence-based detection. The SPR method provides sensitivity comparable to gel electrophoresis, while reducing labor, cross-contamination, and test time, and employs simpler instrumentation with lower cost than real-time PCR.
• We developed a method to detect Aeromonas hydrophila based on surface plasmon resonance (SPR) technology.
• The method has more advantages that include label-free detection, simple operation, multiplex capability, and real-time detection.
• The method with portable SPR equipment can make the field detection of A. hydrophila more convenient.
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Details
- Title
- Development of a surface plasmon resonance (SPR) assay for rapid detection of Aeromonas hydrophila
- Creators
- Linyi Bai - Shandong Normal UniversityHao Zhang - Beijing University of Chemical TechnologyYuan Zhou - Shandong Normal UniversityHongkun Liang - Shandong Normal UniversityShujun Chen - Shandong Normal UniversityXuehui Pang - Shandong Normal UniversityMichael G Mauk - Drexel UniversityLulu Zhang - Beijing University of Chemical TechnologyLei Chen - Shandong Normal University
- Publication Details
- Analytical biochemistry, v 670, 115151
- Publisher
- Elsevier Inc
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Technology
- Web of Science ID
- WOS:000979694900001
- Scopus ID
- 2-s2.0-85151722834
- Other Identifier
- 991021861180904721
UN Sustainable Development Goals (SDGs)
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Chemistry, Analytical