Journal article
Development of an ultrasound sensitive oxygen carrier for oxygen delivery to hypoxic tissue
International journal of pharmaceutics, v 478(1), pp 361-367
18 Nov 2014
PMID: 25448552
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Radiation therapy is frequently used in the treatment of malignancies, but tumors are often more resistant than the surrounding normal tissue to radiation effects, because the tumor microenvironment is hypoxic. This manuscript details the fabrication and characterization of an ultrasound-sensitive, injectable oxygen microbubble platform (SE61
O2
) for overcoming tumor hypoxia. SE61
O2
was fabricated by first sonicating a mixture of Span 60 and water-soluble vitamin E purged with perfluorocarbon gas. SE61
O2
microbubbles were separated from the foam by flotation, then freeze dried under vacuum to remove all perfluorocarbon, and reconstituted with oxygen. Visually, SE61
O2
microbubbles were smooth, spherical, with an average diameter of 3.1 μm and were reconstituted to a concentration of 6.5 E7 microbubbles/ml. Oxygen-filled SE61
O2
provides 16.9 ± 1.0 dB of enhancement at a dose of 880 μl/l (5.7 E7 microbubbles/l) with a half-life under insonation of approximately 15min. In
in vitro
release experiments, 2 ml of SE61
O2
(1.3 E8 microbubbles) triggered with ultrasound was found to elevate oxygen partial pressures of 100 ml of degassed saline 13.8 mmHg more than untriggered bubbles and 20.6 mmHg more than ultrasound triggered nitrogen-filled bubbles. In preliminary
in vivo
delivery experiments, triggered SE61
O2
resulted in a 30.4 mmHg and 27.4 mmHg increase in oxygen partial pressures in two breast tumor mouse xenografts.
Metrics
Details
- Title
- Development of an ultrasound sensitive oxygen carrier for oxygen delivery to hypoxic tissue
- Creators
- John R. Eisenbrey - Thomas Jefferson UniversityLorenzo Albala - Drexel UniversityMichael R. Kramer - Temple UniversityNick Daroshefski - Drexel UniversityDavid Brown - Drexel UniversityJi-Bin Liu - Thomas Jefferson UniversityMaria Stanczak - Thomas Jefferson UniversityPatrick O’Kane - Department of Radiology, Thomas Jefferson University, 132 South 10th Street, Philadelphia, PA 19107, USA School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, USA Temple University School of Medicine, Temple University, Philadelphia, PA 19140, USAFlemming Forsberg - Thomas Jefferson UniversityMargaret A. Wheatley - Drexel University
- Publication Details
- International journal of pharmaceutics, v 478(1), pp 361-367
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000348621100040
- Scopus ID
- 2-s2.0-84918816622
- Other Identifier
- 991019168695704721
UN Sustainable Development Goals (SDGs)
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Pharmacology & Pharmacy