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Journal article
Developmental Expression of Two Spore Wall Proteins during Maturation of the Microsporidian Encephalitozoon intestinalis
Infection and immunity, v 69(11), pp 7057-7066
01 Nov 2001
PMID: 11598081
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Microsporidia are intracellular eukaryotes that infect many animals and cause opportunistic infections in AIDS patients. The disease is transmitted via environmentally resistant spores. Two spore wall constituents from the microsporidian
Encephalitozoon intestinalis
were characterized. Spore wall protein 1 (SWP1), a 50-kDa glycoprotein recognized by monoclonal antibody (MAb) 11B2, was detected in developing sporonts and at low levels on the surfaces of mature spores. In contrast, SWP2, a 150-kDa glycoprotein recognized by MAb 7G7, was detected on fully formed sporonts and was more abundant on mature spores than SWP1. Nevertheless, the SWPs appeared to be complexed on the surfaces of mature spores. SWP1 and SWP2 are similar at the DNA and protein levels and have 10 conserved cysteines in the N-terminal domain, suggesting similar secondary structures. The C-terminal domain of SWP2 has a unique region containing 50 repeating 12- or 15-amino-acid units that lacks homology to known protein motifs. Antibodies from mice infected with
E. intestinalis
recognized SWP1 and SWP2. The characterization of two immunogenic SWPs from
E. intestinalis
will allow the study of exospore structure and function and may lead to the development of useful tools in the diagnosis and treatment of microsporidiosis.
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Details
- Title
- Developmental Expression of Two Spore Wall Proteins during Maturation of the Microsporidian Encephalitozoon intestinalis
- Creators
- J. Russell Hayman - National Institute of Allergy and Infectious DiseasesStanley F. Hayes - National Institute of Allergy and Infectious DiseasesJoseph Amon - Uniformed Services University of the Health SciencesTheodore E. Nash - National Institute of Allergy and Infectious Diseases
- Publication Details
- Infection and immunity, v 69(11), pp 7057-7066
- Publisher
- American Society for Microbiology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Community Health and Prevention
- Web of Science ID
- WOS:000171739200059
- Scopus ID
- 2-s2.0-0034778159
- Other Identifier
- 991021895682104721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology
- Infectious Diseases