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Journal article
Developmental changes in embryonic hypothalamic neurons during prenatal fat exposure
American journal of physiology: endocrinology and metabolism, v 303(3), pp E432-E441
01 Aug 2012
PMID: 22693204
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Poon K, Barson JR, Fagan SE, Leibowitz SF. Developmental changes in embryonic hypothalamic neurons during prenatal fat exposure. Am J Physiol Endocrinol Metab 303: E432-E441, 2012. First published June 12, 2012; doi:10.1152/ajpendo.00238.2012.-Maternal consumption of a fat-rich diet during pregnancy, which causes later overeating and weight gain in offspring, has been shown to stimulate neurogenesis and increase hypothalamic expression of orexigenic neuropeptides in these postnatal offspring. The studies here, using an in vitro model that mimics in vivo characteristics after prenatal high-fat diet (HFD) exposure, investigate whether these same peptide changes occur in embryos and if they are specific to neurons. Isolated hypothalamic neurons were compared with whole hypothalamus from embryonic day 19 (E19) embryos that were prenatally exposed to HFD and were both found to show similar increases in mRNA expression of enkephalin (ENK) and neuropeptide Y (NPY) compared with that of chow-exposed embryos, with no change in melanin-concentrating hormone, orexin, or galanin. Further examination using immunofluorescence cytochemistry revealed an increase in the number of cells expressing ENK and NPY. By plotting the fluorescence intensity of each cell as a probability density function, three different populations of neurons with low, medium, or high levels of ENK or NPY were found in both HFD and chow groups. The prenatal HFD shifted the density of neurons from the population containing low peptide levels to the population containing high peptide levels. This study indicates that neuronal culture is a useful in vitro system for studying diet effects on neuronal development and shows that prenatal HFD exposure alters the population of hypothalamic neurons containing ENK and NPY in the embryo. These changes may contribute to the increase in HFD intake and body weight observed in offspring.
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Details
- Title
- Developmental changes in embryonic hypothalamic neurons during prenatal fat exposure
- Creators
- Kinning Poon - Rockefeller UniversityJessica R. Barson - Rockefeller UniversityShawn E. Fagan - Rockefeller UniversitySarah F. Leibowitz - Rockefeller University
- Publication Details
- American journal of physiology: endocrinology and metabolism, v 303(3), pp E432-E441
- Publisher
- Amer Physiological Soc
- Number of pages
- 10
- Grant note
- UL1TR000043 / NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Advancing Translational Sciences (NCATS) R01DA021518 / NATIONAL INSTITUTE ON DRUG ABUSE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Drug Abuse (NIDA); European Commission 8 UL1 TR000043 / Translation Technology Core Laboratory (CTSA, RUCCTS) from the National Center for Advancing Translational Sciences 5R01DA-021518 / National Institute on Drug Abuse Grant
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; College of Medicine; Drexel University
- Web of Science ID
- WOS:000307228700013
- Scopus ID
- 2-s2.0-84864529546
- Other Identifier
- 991020099965504721
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InCites Highlights
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- Web of Science research areas
- Endocrinology & Metabolism
- Physiology