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Developmental expression of Wnt signaling factors in mouse brain
Journal article   Open access   Peer reviewed

Developmental expression of Wnt signaling factors in mouse brain

Jacquelyn Coyle-Rink, Luis Del Valle, Thersa Sweet, Kamel Khalili and Shohreh Amini
Cancer biology & therapy, v 1(6), pp 640-645
01 Nov 2002
DOI: https://doi.org/10.4161/cbt.313
PMID: 12642687
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.4161/cbt.313View
Published, Version of Record (VoR) Open

Abstract

Animals beta Catenin Blotting, Western Brain - growth & development Brain - metabolism Cell Nucleus Cyclin D1 - metabolism Cytoplasm Cytoskeletal Proteins - metabolism DNA-Binding Proteins - metabolism Gene Expression Regulation, Developmental Glycogen Synthase Kinase 3 - metabolism Immunoenzyme Techniques Lymphoid Enhancer-Binding Factor 1 Mice Myelin Basic Protein - metabolism Protein-Tyrosine Kinases - metabolism Proto-Oncogene Proteins - metabolism Proto-Oncogene Proteins c-myc - metabolism Signal Transduction TCF Transcription Factors Trans-Activators - metabolism Transcription Factor 7-Like 2 Protein Transcription Factors - metabolism Wnt Proteins Zebrafish Proteins
The Wnt signaling pathway has been implicated in a variety of biological events inducing neurogenesis. In this study, we aim to investigate the expression pattern of various components of the Wnt pathway including b-catenin and its partners LEF-1/TCF-4, GSK-3beta and their nuclear target genes such as c-myc and cyclin D1 during mouse brain development. We performed a series of Western blot and immunohistochemistry of brain cortex, brainstem, and cerebellum which revealed differential accumulation of these proteins in different types of brain cells including neurons, astrocytes, and oligodendrocytes at different developmental stages. Intense cytoplasmic immunolabeling of beta-catenin in 5 day old neurons throughout the cortex and brainstem significantly decreased as the brain developed, whereas the level of GSK-3beta, the protein that phosphorylates beta-catenin and causes its destabilization, increased during brain maturation. On the other hand, high level accumulation of LEF-1 and TCF-4 in neurons and astrocytes at the early stage of brain development diminished at the later stages. Interestingly, while the majority of LEF-1 and TCF-4 immunoreactivity was detected in the cytoplasm of neurons, it was evident that both proteins accumulated in the nuclei of astrocytes. Examination of cyclin D1, a protein that controls the cell cycle and proliferation, exhibited an intense staining in the nuclei of astrocytes throughout brain parenchyma during development. Interestingly, cyclin D was found in the cytoplasm of neurons from cortex, brainstem, and cerebellum during brain development. These data provide compelling evidence for the differential expression of the Wnt signaling pathway during brain development, and suggest that these signaling pathways may function differently in various brain regions and cell types.

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Web of Science research areas
Oncology
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