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Developmental regulation of GAP-43, glutamine synthetase and β-actin mRNA in rat cortical astrocytes
Journal article   Peer reviewed

Developmental regulation of GAP-43, glutamine synthetase and β-actin mRNA in rat cortical astrocytes

Anna da Cunha, Vincent J. Aloyo and Ljubiša Vitković
Brain research. Developmental brain research, v 64(1), pp 212-215
1991
DOI: https://doi.org/10.1016/0165-3806(91)90228-B
PMID: 1686218
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Actin mRNA Astrocyte Development GAP-43 Glutamine synthetase
Steady-state levels of mRNA encoding growth-associated protein 43 (GAP-43), glutamine synthetase (GS) and β-actin were measured during development of neonatal rat cortical astrocytes in primary culture. GAP-43 mRNA and protein decreased rapidly during the first 2 weeks and slowly thereafter. In contrast, GS mRNA increased approximately 3-fold during the first 2 weeks and reached maximum by day 15. Actin mRNA first increased up to 8 days and decreased thereafter reaching a constant amount by 15 days, similar to the initial low value. Thus, GAP-43, GS and β-actin mRNA levels are differentially regulated during development of astrocytes in primary culture. Because the patterns of expression of astrocytic markers GS and GFAP (shown previously) in vitro and in vivo are similar to each other, primary cultures of astrocytes may be an excellent system for investigating mechanisms of developmental regulation of these genes.

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Collaboration types
Domestic collaboration
Web of Science research areas
Developmental Biology
Neurosciences
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