Files and links (1)
Published, Version of Record (VoR)CC BY V4.0, Open
Journal article
Dextran and Polymer Polyethylene Glycol (PEG) Coating Reduce Both 5 and 30 nm Iron Oxide Nanoparticle Cytotoxicity in 2D and 3D Cell Culture
International journal of molecular sciences, v 13(5), pp 5554-5570
01 May 2012
PMID: 22754315
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Superparamagnetic iron oxide nanoparticles are widely used in biomedical applications, yet questions remain regarding the effect of nanoparticle size and coating on nanoparticle cytotoxicity. In this study, porcine aortic endothelial cells were exposed to 5 and 30 nm diameter iron oxide nanoparticles coated with either the polysaccharide, dextran, or the polymer polyethylene glycol (PEG). Nanoparticle uptake, cytotoxicity, reactive oxygen species (ROS) formation, and cell morphology changes were measured. Endothelial cells took up nanoparticles of all sizes and coatings in a dose dependent manner, and intracellular nanoparticles remained clustered in cytoplasmic vacuoles. Bare nanoparticles in both sizes induced a more than 6 fold increase in cell death at the highest concentration (0.5 mg/mL) and led to significant cell elongation, whereas cell viability and morphology remained constant with coated nanoparticles. While bare 30 nm nanoparticles induced significant ROS formation, neither 5 nm nanoparticles (bare or coated) nor 30 nm coated nanoparticles changed ROS levels. Furthermore, nanoparticles were more toxic at lower concentrations when cells were cultured within 3D gels. These results indicate that both dextran and PEG coatings reduce nanoparticle cytotoxicity, however different mechanisms may be important for different size nanoparticles.
Metrics
Details
- Title
- Dextran and Polymer Polyethylene Glycol (PEG) Coating Reduce Both 5 and 30 nm Iron Oxide Nanoparticle Cytotoxicity in 2D and 3D Cell Culture
- Creators
- Miao Yu - Drexel UniversityShaohui Huang - Univ Penn, Inst Environm Med, Philadelphia, PA 19104 USAKevin Jun Yu - Univ Penn, Inst Environm Med, Philadelphia, PA 19104 USAAlisa Sharon Morss Clyne - Drexel UniversityAlisa Morss Clyne - University of Maryland, College Park
- Publication Details
- International journal of molecular sciences, v 13(5), pp 5554-5570
- Publisher
- Mdpi
- Number of pages
- 17
- Grant note
- University of Pennsylvania Center of Excellence in Environmental Toxicology Nanotechnology Institute Pilot Projects in Nanotoxicology CMMI-1038769 / National Science Foundation; National Science Foundation (NSF) 1038769 / Directorate For Engineering; Div Of Civil, Mechanical, & Manufact Inn; National Science Foundation (NSF); NSF - Directorate for Engineering (ENG)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000306186200019
- Scopus ID
- 2-s2.0-84861537584
- Other Identifier
- 991022005887604721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary