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Journal article
Differences in testosterone and its precursors by sex of the offspring in meconium
The Journal of steroid biochemistry and molecular biology, v 167, pp 78-85
01 Mar 2017
PMID: 27871978
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Prenatal metabolism exerts profound effects on development. The first stool of the newborn, meconium, provides a window into the prenatal metabolic environment. The objective of this study was to examine the feasibility of meconium as a novel matrix to quantify prenatal steroid levels. We quantified parameters of analytical interest regarding the use of meconium, including sample stability. We hypothesized that meconium steroid content would differ by sex, prompting analysis of meconium to test effects of prenatal steroid metabolism. Meconium from 193 newborns enrolled in the Early Autism Risk Longitudinal Investigation (EARLI) study, including 107 males, and 86 females, were analyzed by isotope dilution-liquid chromatography-high resolution mass spectrometry (ID-LC-HRMS) while blinded to identity for testosterone (T), androstenedione (AD), and dehydroepiandrosterone (DHEA). Steroid levels were compared by sex, and investigations of potential trends resulting from sample storage or processing was conducted. The unconjugated steroid content of meconiuni in ngig (mean, standard deviation) was for males: T (2.67, 8.99), AD (20.01, 28.12), DHEA (13.96, 23.57) and for females: T (0.82, 1.63), AD (22.32, 24.38), DHEA (21.06, 43.49). T was higher in meconium from males (p= 0.0333), and DHEA was higher in meconium from females (p = 0.0202). 6 female and 3 male T values were below the limit of detection. No extreme variability in hydration or trend in steroid levels by storage time was detected. Sexually dimorphic levels of hormones may reflect gestational differentiation, and future studies should consider meconium analysis. (C) 2016 Elsevier Ltd. All rights reserved.
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Details
- Title
- Differences in testosterone and its precursors by sex of the offspring in meconium
- Creators
- Alexander J. Frey - Drexel UniversityBo Y. Park - BloombergEmily R. Schriver - Drexel UniversityDaniel R. Feldman - Drexel UniversitySamuel Parry - University of PennsylvaniaLisa A. Croen - Kaiser PermanenteDaniele M. Fallin - Johns Hopkins Bloomberg Sch Publ Hlth, Dept Mental Hlth, Wendy Klag Ctr Autism & Dev Disabil, 624N Broadway,HH 850, Baltimore, MD 21205 USAIrva Hertz-Picciotto - MIND InstituteCraig J. Newschaffer - Drexel UniversityNathaniel W. Snyder - Drexel University
- Publication Details
- The Journal of steroid biochemistry and molecular biology, v 167, pp 78-85
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- R03CA211820 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) R01 ES016443; K22ES26235; R21HD087866; R21ES025559 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R21ES025559 / NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS) 9502; 8556 / Autism Speaks R21HD087866 / EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- A.J. Drexel Autism Institute
- Web of Science ID
- WOS:000394079300010
- Scopus ID
- 2-s2.0-85007042125
- Other Identifier
- 991019168052104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Endocrinology & Metabolism