Journal article
Differential Expression and Cellular Distribution of gamma-Tubulin and beta III-Tubulin in Medulloblastomas and Human Medulloblastoma Cell Lines
Journal of cellular physiology, v 223(2), pp 519-529
01 May 2010
PMID: 20162618
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In previous studies, we have shown overexpression and ectopic subcellular distribution of gamma-tubulin and beta III-tubulin in human glioblastomas and glioblastoma cell lines (Katsetos et al., 2006,] Neuropathol Exp Neurol 65:455-467; Katsetos et al., 2007, Neurochem Res 32:1387-1398). Here we determined the expression of gamma-tubulin in surgically excised medulloblastomas (n = 20) and in the human medulloblastoma cell lines D283 Med and DAOY. In clinical tissue samples, the immunohistochemical distribution of gamma-tubulin labeling was pervasive and inversely related to neuritogenesis. Overexpression of gamma-tubulin was widespread in poorly differentiated, proliferating tumor cells but was significantly diminished in quiescent differentiating tumor cells undergoing neuritogenesis, highlighted by beta III-tubulin immunolabeling. By quantitative real-time PCR, gamma-tubulin transcripts for TUBG1, TUBG2, and TUBB3 genes were detected in both cell lines but expression was less prominent when compared with the human glioblastoma cell lines. Immunoblotting revealed comparable amounts of gamma-tubulin and beta III-tubulin in different phases of cell cycle; however, a larger amount of gamma-tubulin was detected in D283 Med when compared with DAOY cells. Interphase D283 Med cells exhibited predominantly diffuse cytoplasmic gamma-tubulin localization, in addition to the expected centrosome-associated distribution. Robust beta III-tubulin immunoreactivity was detected in mitotic spindles of DAOY cells. Our data indicate that overexpression of gamma-tubulin may be linked to phenotypic dedifferentiation (anaplasia) and tumor progression in medulloblastomas and may potentially serve as a promising tumor marker. J. Cell. Physiol. 223: 519-529,2010. (C) 2010 Wiley-Liss, Inc.
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Details
- Title
- Differential Expression and Cellular Distribution of gamma-Tubulin and beta III-Tubulin in Medulloblastomas and Human Medulloblastoma Cell Lines
- Creators
- Valentina Caracciolo - Temple UniversityLuca D'Agostino - Temple UniversityEduarda Draberova - Cytoskeleton (United States)Vladimira Sladkova - Cytoskeleton (United States)Catena Crozier-Fitzgerald - Temple UniversityDimitri P. Agamanolis - Northeast Ohio Medical UniversityJean-Pierre De Chadarevian - St. Christopher's Hospital for ChildrenAgustin Legido - St. Christopher's Hospital for ChildrenAntonio Giordano - Temple UniversityPavel Draber - Cytoskeleton (United States)Christos D. Katsetos - St. Christopher's Hospital for Children
- Publication Details
- Journal of cellular physiology, v 223(2), pp 519-529
- Publisher
- Wiley
- Number of pages
- 11
- Grant note
- 204/09/1777 / Grant Agency of the Czech Republic AVOZ 50520514 / Institutional Research Support St. Christopher's Foundation for Children Human Health Foundation Sbarro Health Research Organization (S.H.R.O.) 1M683780500 / Ministry of Education, Youth and Sports of the Czech Republic; Ministry of Education, Youth & Sports - Czech Republic KAN200520701 / GA AVCR; Grant Agency of the Czech Republic
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000276428100028
- Scopus ID
- 2-s2.0-77950842886
- Other Identifier
- 991019353726104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology
- Physiology