Journal article
Differential association of programmed death-1 and CD57 with ex vivo survival of CD8+ T cells in HIV infection
The Journal of immunology (1950), v 183(2), pp 1120-1132
15 Jul 2009
PMID: 19564339
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Recent studies have revealed the critical role of programmed death-1 (PD-1) in exhaustion of HIV- and SIV-specific CD8(+) T cells. In this study, we show that high expression of PD-1 correlates with increased ex vivo spontaneous and CD95/Fas-induced apoptosis, particularly in the "effector-memory" CD8(+) T cell population from HIV(+) donors. High expression of PD-1 was linked to a proapoptotic phenotype characterized by low expression of Bcl-2 and IL7-R alpha, high expression of CD95/Fas and high mitochondrial mass. Expression of PD-1 and CD57 was differentially associated with the maturation status of CD8(+) T cells in HIV infection. CD57 was linked to higher apoptosis resistance, with cells expressing a PD-1(L)CD57(H) phenotype exhibiting lower levels of cell death. The majority of HIV-specific CD8(+) T cells were found to express a PD-1(H)CD57(L) or PD-1(H)CD57(H) phenotype. No correlation was found between PD-1 expression and ex vivo polyfunctionality of either HIV- or CMV-specific CD8(+) T cells. Contrary to CD57, high expression of PD-1 was characterized by translocation of PD-1 into the area of CD95/Fas-capping, an early necessary step of CD95/Fas-induced apoptosis. Thus, our data further support the role of PD-1 as a preapoptotic factor for CD8(+) T cells in HIV infection.
Metrics
Details
- Title
- Differential association of programmed death-1 and CD57 with ex vivo survival of CD8+ T cells in HIV infection
- Creators
- Constantinos Petrovas - National Institute of Allergy and Infectious DiseasesBenjamin Chaon - National Institutes of HealthDavid R Ambrozak - National Institutes of HealthDavid A Price - Cardiff UniversityJ Joseph Melenhorst - National Institutes of HealthBrenna J Hill - National Institutes of HealthChristof Geldmacher - National Institutes of HealthJoseph P Casazza - National Institutes of HealthPratip K Chattopadhyay - National Institutes of HealthMario Roederer - National Institutes of HealthDaniel C Douek - National Institutes of HealthYvonne M Mueller - Drexel UniversityJeffrey M Jacobson - Drexel UniversityViraj Kulkarni - National Institutes of HealthBarbara K Felber - National Institutes of HealthGeorge N Pavlakis - National Institutes of HealthPeter D Katsikis - Drexel UniversityRichard A Koup - National Institutes of Health
- Publication Details
- The Journal of immunology (1950), v 183(2), pp 1120-1132
- Publisher
- American Association of Immunologists (AAI)
- Grant note
- R01 AI046719-10 / NIAID NIH HHS R01 AI046719 / NIAID NIH HHS Z99 AI999999 / Intramural NIH HHS G0501963 / Medical Research Council R01 AI46719 / NIAID NIH HHS
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000267812600038
- Scopus ID
- 2-s2.0-70249122418
- Other Identifier
- 991019335513804721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Immunology