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Differential effects of acute gonadal steroid treatment on neuronal survival in the brainstem following C3 hemicontusion
Journal article   Open access   Peer reviewed

Differential effects of acute gonadal steroid treatment on neuronal survival in the brainstem following C3 hemicontusion

Gina N Monaco, Talat A Khan, Kengo Ogata, John D Houle and Kathryn J Jones
The FASEB journal, v 24(S1), pp 640.3-640.3
Apr 2010
DOI: https://doi.org/10.1096/fasebj.24.1_supplement.640.3
url
https://doi.org/10.1096/fasebj.24.1_supplement.640.3View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze),  Open

Abstract

Following gonadectomy, adult male and female Sprague‐Dawley rats underwent a cervical level 3 hemicontusion spinal cord injury (C3HC SCI) and were randomized to receive testosterone (T), estrogen (E), or sham pellet, and True Blue (TB)‐soaked gelfoam was placed into the lesion cavity to retrogradely label injured supraspinal neurons. At 4 weeks post‐C3HC, brains and cervical spinal cords were removed, sectioned, fixed, mounted, and examined with fluorescence microscopy for TB‐labeled neurons. Neurons in the red nucleus and lateral vestibular nuclei were counted from alternating sections. For the lateral vestibular neurons: sham‐treated males exhibited greater baseline cell survival than did females following injury, T had a positive effect on cell survival in female animals, but E had a negative effect on cell survival in female animals. In the red nucleus, cell survival was similar between the sham‐treated sexes, T did not have an effect on cell survival in females, and E had a negative effect on cell survival in females. The results suggest there are sex differences in the response to injury in different brain stem nuclei, as well as differential and regional effects of T versus E treatments on cell survival. This work was supported by VA Rehab R&D Merit Review Project # B4952I (KJ Jones & JD Houle). Grant Funding Source: VA Rehab R&D Merit Review Project # B4952I (KJ Jones & JD Houle)

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