Journal article
Differential effects of ethanol on signal transduction
Alcoholism, clinical and experimental research, v 24(1), pp 93-101
01 Jan 2000
PMID: 10656198
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background: PC12 pheochromocytoma cells were used as a model to study the effect of long-term ethanol exposure on signal transduction systems. In PC12 cells, the agonist bradykinin stimulates a phospholipase C specific for inositol-containing phospholipids and a phospholipase D specific for phosphatidylcholine.
Methods: PC12 cells were grown in monolayer and cultured in the presence and absence of 1% ethanol for 5 days. After this period, bradykinin-stimulated phospholipase C and D were measured. The effect of long-term ethanol on the bradykinin-mediated activation of mitogen-activated protein (MAP) kinase was also measured.
Results: In cells exposed to 1% ethanol for 5 days, bradykinin-stimulated phospholipase D was greatly attenuated, whereas bradykinin-stimulated phospholipase C was not altered. The tyrosine kinase inhibitor, genistein, blocked the bradykinin-mediated activation of phospholipase D but did not affect the stimulation of phospholipase C. However, long-term ethanol treatment did not attenuate the ability of bradykinin to activate MAP kinase, which suggests that ethanol did not have a general effect on all tyrosine kinase pathways.
Conclusions: Ethanol has a differential effect on signal transduction in PC12 cells. Activation of phospholipase D may be mediated by a kinase, whereas the activation of phospholipase C is probably mediated by the guanine nucleotide binding protein, Gq. Because of these differences in activation mechanism, the pathways may adapt differently to long-term exposure to ethanol.
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Details
- Title
- Differential effects of ethanol on signal transduction
- Creators
- G H LevineJ J MaglioJ Horwitz
- Publication Details
- Alcoholism, clinical and experimental research, v 24(1), pp 93-101
- Publisher
- Wiley
- Number of pages
- 9
- Grant note
- AA10070 / NIAAA NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Alcohol Abuse & Alcoholism (NIAAA) R03AA010070 / NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Alcohol Abuse & Alcoholism (NIAAA)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000084901900014
- Scopus ID
- 2-s2.0-0033985586
- Other Identifier
- 991019169685004721
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