Journal article
Differential effects of the complement peptides, C5a and C5a des Arg on human basophil and lung mast cell histamine release
The Journal of clinical investigation, v 81(3), pp 918-923
01 Mar 1988
PMID: 2449462
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The ability of purified anaphylatoxins to induce human lung mast cell mediator release was investigated. In eight anti-IgE responsive (histamine release = 22 +/- 5%, mean +/- SEM) mast cell preparations of 1-96% purity, C5a and C5a des Arg (0.55 pg/ml to 55 micrograms/ml), failed to elicit or potentiate histamine release; lung fragments were similarly unresponsive. The related peptide C3a was also inactive. All anaphylatoxins failed to induce mast cell leukotriene C4 (LTC4) and prostaglandin D2 (PGD2) release. LTC4 release was also negligible from basophils where C5a was a potent histamine release stimulus. Supernatants from C5a-challenged mast cells remained fully active on basophils, excluding carboxypeptidase inactivation of C5a as an explanation for the lung mast cell results. In contrast to lung, skin mast cells were C5a-responsive (histamine release = 8 +/- 1%, at 55 micrograms/ml, n = 2). We conclude that C5a, though devoid of activity on the human lung mast cell, is a human basophil and skin mast cell secretagogue. These findings demonstrate significant organ-specific heterogeneity in mast cell responsiveness.
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Details
- Title
- Differential effects of the complement peptides, C5a and C5a des Arg on human basophil and lung mast cell histamine release
- Creators
- E S Schulman - Jefferson College of Health SciencesT J Post - Department of Medicine, Jefferson Medical College, Philadelphia, Pennsylvania 19107P M Henson - Department of Medicine, Jefferson Medical College, Philadelphia, Pennsylvania 19107P C Giclas - Department of Medicine, Jefferson Medical College, Philadelphia, Pennsylvania 19107
- Publication Details
- The Journal of clinical investigation, v 81(3), pp 918-923
- Publisher
- American Society for Clinical Investigation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pulmonary, Critical Care, and Sleep (Medicine)
- Web of Science ID
- WOS:A1988M255000038
- Scopus ID
- 2-s2.0-0023849702
- Other Identifier
- 991019295190404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Medicine, Research & Experimental