Journal article
Differential expression of chaperonin containing T-complex polypeptide (CCT) subunits during fetal and adult skin wound healing
Cell stress & chaperones, v 13(4), pp 527-533
Dec 2008
PMID: 18465209
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Integumentary wound healing in early fetal life is regenerative and proceeds without scar formation. Expressomic analysis of this phenomenon by differential display has previously determined that the eta subunit of the cytosolic chaperonin containing T-complex polypeptide (CCT) is downregulated in the healing fetal wound milieu. We now report that no other CCT subunit shares this distinct pattern of gene regulation as determined by limiting dilution reverse transcriptase polymerase chain reaction (RT-PCR); all seven of the remaining CCT subunits demonstrate no change in messenger RNA (mRNA) expression in healing fetal wounds compared to unwounded control tissue. The alpha subunit, however, did evidence reduced message levels in healing adult wound tissue. We herein report on the cloning and sequence of the complementary DNA (cDNA) for rabbit CCT-alpha and confirm its wound specific decrease in adult tissues through quantitative real-time RT-PCR assay. We also confirm that quantitative evaluation of CCT-alpha and CCT-zeta mRNA expression shows no change in healing fetal wounds.
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Details
- Title
- Differential expression of chaperonin containing T-complex polypeptide (CCT) subunits during fetal and adult skin wound healing
- Creators
- Latha Satish - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212 USAAdam Abdulally - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212 USADuane Oswald - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212 USASandra Johnson - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212 USAFen Ze Hu - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212 USAJ. Christopher Post - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212 USAGarth D Ehrlich - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212 USASandeep Kathju - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212 USA
- Publication Details
- Cell stress & chaperones, v 13(4), pp 527-533
- Publisher
- Springer Netherlands; Dordrecht
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000261302400013
- Scopus ID
- 2-s2.0-59649124624
- Other Identifier
- 991014877831004721
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- Web of Science research areas
- Cell Biology