Journal article
Differential gene expression pattern of diabetic rat retinas after intravitreal injection of erythropoietin
Clinical & experimental ophthalmology, v 39(2), pp 142-151
01 Mar 2011
PMID: 20973890
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Abstract
P>Background:
To profile the pattern of gene expression in diabetic rat retinas with or without intravitreal injection of erythropoietin.
Design:
By using streptozotocin-induced diabetic rats, after intravitreal injection of erythropoietin, neurosensory retinas were collected to determine the effect of erythropoietin on gene expression.
Participants:
Three groups of Sprague-Dawley rats were studied: normal control (15), diabetic rats with saline injection (15) and diabetic rats with intravitreal erythropoietin treatment (15).
Methods:
Diabetes was induced by intra-peritoneal injection of streptozotocin. Intravitreal injection of erythropoietin was performed at the following time points: 0, 30 and 120 days after diabetes onset. Four days after each injection at above-mentioned time points, the retinas were harvested for microarray assay. The real-time PCR was used to evaluate the microarray data.
Results:
Genes encoding inflammatory factors, such as interleukin-2 and interleukin-11, which were upregulated in the diabetic retinas, were restored after erythropoietin treatment. Genes encoding pro-apoptotic effectors, like Tnfrsf5, Bid3 and Bcl2l1, were also upregulated in diabetic rats and attenuated in erythropoietin-treated group. In addition, real-time PCR were employed to confirm the changes of the genes Trex2, G1P2, DHX58, RGD1311906 and LOC689064, which have not been reported in diabetic retinopathy.
Conclusions:
Intravitreal erythropoietin treatment is able to normalize the gene expression responsible for pro-apoptotic and inflammatory responses noted in diabetic retinas.
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Details
- Title
- Differential gene expression pattern of diabetic rat retinas after intravitreal injection of erythropoietin
- Creators
- Qing Chu - Laboratory of Clinical Visual Sciences, Institute of Health Sciences, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.Jingfa Zhang - Tongji UniversityYalan Wu - Laboratory of Clinical Visual Sciences, Institute of Health Sciences, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesYu Zhang - Huashan HospitalGuoxu Xu - Soochow Univ, Affiliated Hosp 2, Dept Ophthalmol, Suzhou, Peoples R ChinaWeiye Li - Drexel UniversityGuo-Tong Xu - Second Affiliated Hospital of Soochow University
- Publication Details
- Clinical & experimental ophthalmology, v 39(2), pp 142-151
- Publisher
- Wiley
- Number of pages
- 10
- Grant note
- 1500219024; 2010QH04; 2010YF02 / Tongji University 08410701200 / Sciences and Technology Commission of Shanghai Municipality Clear Vision Foundation, Philadelphia, USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Ophthalmology [Historical]
- Web of Science ID
- WOS:000288458800009
- Scopus ID
- 2-s2.0-79952684029
- Other Identifier
- 991019167612704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Ophthalmology