Journal article
Differentiated activities of decorin and biglycan in the progression of post-traumatic osteoarthritis
Osteoarthritis and cartilage, v 29(8), pp 1181-1192
Aug 2021
PMID: 33915295
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To delineate the activities of decorin and biglycan in the progression of post-traumatic osteoarthritis (PTOA).
Three-month-old inducible biglycan (BgniKO) and decorin/biglycan compound (Dcn/BgniKO) knockout mice were subjected to the destabilization of the medial meniscus (DMM) surgery to induce PTOA. The OA phenotype was evaluated by assessing joint structure and sulfated glycosaminoglycan (sGAG) staining via histology, surface collagen fibril nanostructure and calcium content via scanning electron microscopy, tissue modulus via atomic force microscopy-nanoindentation, as well as subchondral bone structure and meniscus ossification via micro-computed tomography. Outcomes were compared with previous findings in the inducible decorin (DcniKO) knockout mice.
In the DMM model, BgniKO mice developed similar degree of OA as the control (0.44 [-0.18 1.05] difference in modified Mankin score), different from the more severe OA phenotype observed in DcniKO mice (1.38 [0.91 1.85] difference). Dcn/BgniKO mice exhibited similar histological OA phenotype as DcniKO mice (1.51 [0.97 2.04] difference vs control), including aggravated loss of sGAGs, salient surface fibrillation and formation of osteophyte. Meanwhile, Dcn/BgniKO mice showed further cartilage thinning than DcniKO mice, resulting in the exposure of underlying calcified tissues and aberrantly high surface modulus. BgniKO and Dcn/BgniKO mice developed altered subchondral trabecular bone structure in both Sham and DMM groups, while DcniKO and control mice did not.
In PTOA, decorin plays a more crucial role than biglycan in regulating cartilage degeneration, while biglycan is more important in regulating subchondral bone structure. The two have distinct activities and modest synergy in the pathogenesis of PTOA.
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Details
- Title
- Differentiated activities of decorin and biglycan in the progression of post-traumatic osteoarthritis
- Creators
- B. Han - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA, 19104, United StatesQ. Li - Drexel UniversityC. Wang - Drexel UniversityP. Chandrasekaran - Drexel UniversityY. Zhou - University of TorontoL. Qin - University of PennsylvaniaX.S. Liu - University of PennsylvaniaM. Enomoto-Iwamoto - University of Maryland, BaltimoreD. Kong - University of TorontoR.V. Iozzo - Thomas Jefferson UniversityD.E. Birk - University of South FloridaL. Han - Drexel University
- Publication Details
- Osteoarthritis and cartilage, v 29(8), pp 1181-1192
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Marketing; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000681317800011
- Scopus ID
- 2-s2.0-85106369100
- Other Identifier
- 991019168894704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Orthopedics
- Rheumatology