Journal article
Differentiation of xenografted human fetal lung parenchyma
Early human development, v 84(3)
2008
PMID: 17555893
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The goal of this study was to characterize xenografted human fetal lung tissue with respect to developmental stage-specific cytodifferentiation. Human fetal lung tissue (pseudoglandular stage) was grafted either beneath the renal capsule or the skin of athymic mice (NCr-nu). Tissues were analyzed from 3 to 42 days post-engraftment for morphological alterations by light and electron microscopy (EM), and for surfactant protein mRNA and protein by reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry (ICC), respectively. The changes observed resemble those seen in human lung development
in utero in many respects, including the differentiation of epithelium to the saccular stage. Each stage occurred over approximately one week in the graft in contrast to the eight weeks of normal
in utero development. At all time points examined, all four surfactant proteins (SP-A, SP-B, SP-C, and SP-D) were detected in the epithelium by ICC. Lamellar bodies were first identified by EM in 14 day xenografts. By day 21, a significant increase in lamellar body expression was observed. Cellular proliferation, as marked by PCNA ICC and elastic fiber deposition resembled those of canalicular and saccular
in utero development. This model in which xenografted lung tissue in different stages of development is available may facilitate the study of human fetal lung development and the impact of various pharmacological agents on this process.
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Details
- Title
- Differentiation of xenografted human fetal lung parenchyma
- Creators
- Jelena Pavlovic - Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USAJoanna Floros - Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USADavid S Phelps - Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USABrian Wigdahl - Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USAPatricia Welsh - Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USAJudith Weisz - Department of Obstetrics and Gynecology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USADebra A Shearer - Department of Obstetrics and Gynecology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USAAlphonse Leure du Pree - Department of Neuroscience and Anatomy, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USARoland Myers - Department of Neuroscience and Anatomy, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USAMary K Howett - Department of Obstetrics and Gynecology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
- Publication Details
- Early human development, v 84(3)
- Publisher
- Elsevier Ireland Ltd
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000255434200007
- Scopus ID
- 2-s2.0-41149119451
- Other Identifier
- 991014878638204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Obstetrics & Gynecology
- Pediatrics