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Dihydropyrrolopyrazol-6-one MCHR1 antagonists for the treatment of obesity: Insights on in vivo efficacy from a novel FLIPR assay setup
Journal article   Open access   Peer reviewed

Dihydropyrrolopyrazol-6-one MCHR1 antagonists for the treatment of obesity: Insights on in vivo efficacy from a novel FLIPR assay setup

Pratik Devasthale, Wei Wang, Andres S Hernandez, Fang Moore, Kishore Renduchintala, Radhakrishnan Sridhar, Mary Ann Pelleymounter, Daniel Longhi, Ning Huang, Neil Flynn, …
Bioorganic & medicinal chemistry letters, v 25(14), pp 2793-2799
15 Jul 2015
PMID: 26022839
url
https://doi.org/10.7270/q29c7053View
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Abstract

Animals Anti-Obesity Agents - chemistry Anti-Obesity Agents - pharmacokinetics Anti-Obesity Agents - pharmacology Anti-Obesity Agents - therapeutic use Half-Life Humans Obesity - drug therapy Protein Binding Pyrazoles - chemistry Pyrazoles - pharmacokinetics Pyrazoles - pharmacology Pyrazoles - therapeutic use Rats Rats, Sprague-Dawley Receptors, Somatostatin - antagonists & inhibitors Receptors, Somatostatin - metabolism Structure-Activity Relationship Weight Loss - drug effects
Our investigation of the structure-activity and structure-liability relationships for dihydropyrrolopyrazol-6-one MCHR1 antagonists revealed that off-rate characteristics, inferred from potencies in a FLIPR assay following a 2 h incubation, can impact in vivo efficacy. The in vitro and exposure profiles of dihydropyrrolopyrazol-6-ones 1b and 1e were comparable to that of the thienopyrimidinone counterparts 41 and 43 except for a much faster MCHR1 apparent off-rate. The greatly diminished dihydropyrrolopyrazol-6-one anti-obesity response may be the consequence of this rapid off-rate.

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7 citations in Scopus

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Collaboration types
Industry collaboration
Domestic collaboration
International collaboration
Web of Science research areas
Chemistry, Medicinal
Chemistry, Organic
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