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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (165)
01 Nov 2020
PMID: 33191938
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Bioprinting is emerging as a promising tool to fabricate 3D human cancer models that better recapitulate critical hallmarks of in vivo tissue architecture. In current layer-by-layer extrusion bioprinting, individual cells are extruded in a bioink together with complex spatial and temporal cues to promote hierarchical tissue self-assembly. However, this biofabrication technique relies on complex interactions among cells, bioinks and biochemical and biophysical cues. Thus, self-assembly may take days or even weeks, may require specific bioinks, and may not always occur when there is more than one cell type involved. We therefore developed a technique to directly bioprint pre-formed 3D breast epithelial spheroids in a variety of bioinks. Bioprinted pre-formed 3D breast epithelial spheroids sustained their viability and polarized architecture after printing. We additionally printed the 3D spheroids onto vascular endothelial cell networks to create a co-culture model. Thus, the novel bioprinting technique rapidly creates a more physiologically relevant 3D human breast model at lower cost and with higher flexibility than traditional bioprinting techniques. This versatile bioprinting technique can be extrapolated to create 3D models of other tissues in additional bioinks.
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Details
- Title
- Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks
- Creators
- Swathi Swaminathan - Drexel UniversityAlisa Morss Clyne - University of Maryland, College Park
- Publication Details
- JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (165)
- Publisher
- Journal Of Visualized Experiments
- Number of pages
- 14
- Grant note
- 1R01HL140239-01 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000596845500019
- Scopus ID
- 2-s2.0-85096266232
- Other Identifier
- 991019167474904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology