A method to directly measure the intracellular pressure of adherent, migrating cells is described in the Basic Protocol. This approach is based on the servo-null method where a microelectrode is introduced into the cell to directly measure the physical pressure of the cytoplasm. We also describe the initial calibration of the microelectrode as well as the application of the method to cells migrating inside three-dimensional (3D) extracellular matrix (ECM).
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Title
Direct Measurement of Intracellular Pressure
Creators
Ryan J. Petrie - National Institute of Dental and Craniofacial Research
Hyun Koo - University of Pennsylvania
Publication Details
Current protocols in cell biology, v 63(1), pp 12.9.1-12.9.9