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Direct Measurement of Intracellular Pressure
Journal article   Open access

Direct Measurement of Intracellular Pressure

Ryan J. Petrie and Hyun Koo
Current protocols in cell biology, v 63(1), pp 12.9.1-12.9.9
03 Jun 2014
PMID: 24894836
url
https://europepmc.org/articles/pmc4075330View
Accepted (AM)Open Access (License Unspecified) Open

Abstract

extracellular matrix fibroblasts Intracellular pressure motility
A method to directly measure the intracellular pressure of adherent, migrating cells is described in the Basic Protocol. This approach is based on the servo-null method where a microelectrode is introduced into the cell to directly measure the physical pressure of the cytoplasm. We also describe the initial calibration of the microelectrode as well as the application of the method to cells migrating inside three-dimensional (3D) extracellular matrix (ECM).

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