Journal article
Direct Measurement of Intracellular Pressure
Current protocols in cell biology, v 63(1), pp 12.9.1-12.9.9
03 Jun 2014
PMID: 24894836
Abstract
A method to directly measure the intracellular pressure of adherent, migrating cells is described in the Basic Protocol. This approach is based on the servo-null method where a microelectrode is introduced into the cell to directly measure the physical pressure of the cytoplasm. We also describe the initial calibration of the microelectrode as well as the application of the method to cells migrating inside three-dimensional (3D) extracellular matrix (ECM).
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30 citations in Scopus
Details
- Title
- Direct Measurement of Intracellular Pressure
- Creators
- Ryan J. Petrie - National Institute of Dental and Craniofacial ResearchHyun Koo - University of Pennsylvania
- Publication Details
- Current protocols in cell biology, v 63(1), pp 12.9.1-12.9.9
- Publisher
- Wiley
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Scopus ID
- 2-s2.0-84940266250
- Other Identifier
- 991020100087904721