Journal article
Direct cell writing of 3D microorgan for in vitro pharmacokinetic model
Tissue engineering. Part C, Methods, v 14(2)
01 Jun 2008
PMID: 18544030
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A novel targeted application of tissue engineering is the development of an in vitro pharmacokinetic model for drug screening and toxicology. An in vitro pharmacokinetic model is needed to realistically and reliably predict in vivo human response to drug administrations and potential toxic exposures. This paper details the fabrication process development and adaptation of microfluidic devices for the creation of such a physiologically relevant pharmacokinetic model. First, an automated syringe-based, layered direct cell writing (DCW) bioprinting process creates a 3D microorgan that biomimics the cell's naturalmicroenvironment with enhanced functionality. Next, soft lithographic micropatterning techniques are used to fabricate a microscale in vitro device to house the 3D microorgan. This paper demonstrates the feasibility of the DCW process for freeform biofabrication of 3D cell-encapsulated hydrogel-based tissue constructs with defined reproducible patterns, direct integration of 3D constructs onto a microfluidic device for continuous perfusion drug flow, and characterization of 3D tissue constructs with predictable cell viability/proliferation outcomes and enhanced functionality over traditional culture methods.
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Details
- Title
- Direct cell writing of 3D microorgan for in vitro pharmacokinetic model
- Creators
- Robert Chang - Drexel UniversityYae Nam - Drexel Univ, Dept Mech Engn & Mech, Philadelphia, PA 19104 USAWei Sun - Drexel Univ, Dept Mech Engn & Mech, Philadelphia, PA 19104 USA
- Publication Details
- Tissue engineering. Part C, Methods, v 14(2)
- Publisher
- Mary Ann Liebert, Inc
- Number of pages
- 10
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000256675800007
- Scopus ID
- 2-s2.0-45249122800
- Other Identifier
- 991019167464604721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell & Tissue Engineering
- Cell Biology
- Engineering, Biomedical
- Materials Science, Biomaterials