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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Direct evidence that HSV DNA damaged by ultraviolet (UV) irradiation can be repaired in a cell type-dependent manner
Journal of neurovirology, v 18(3)
01 Jun 2012
PMID: 22581427
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Infection of permissive cells, in tissue culture, with herpes simplex virus (HSV) has been reported to induce host DNA damage repair responses that are necessary for efficient viral replication. However, direct repair of the damaged viral DNA has not, to our knowledge, been shown. In this report, we detect and determine the amount of damaged HSV-1 DNA, following introduction of experimentally damaged HSV genomes into tissue cultures of permissive Vero, NGF differentiated PC12 cells and primary rat neurons, using a method of detection introduced here. The results show that HSV-1 strain 17 DNA containing UV-induced DNA damage is efficiently repaired, in Vero, but not NGF differentiated PC12 cells. The primary rat neuronal cultures were capable of repairing the damaged viral DNA, but with much less efficiency than did the permissive Vero cells. Moreover, by conducting the experiments with either an inhibitor of the HSV polymerase (phosphonoacetic acid [PAA]) or with a replication defective DNA polymerase mutant virus, HP66, the results suggest that repair can occur in the absence of a functional viral polymerase, although polymerase function seems to enhance the efficiency of the repair, in a replication independent manner. The possible significance of varying cell type mediated repair of viral DNA to viral pathogenesis is discussed.
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Details
- Title
- Direct evidence that HSV DNA damaged by ultraviolet (UV) irradiation can be repaired in a cell type-dependent manner
- Creators
- Scott Millhouse - Drexel UniversityXiaohe Wang - Drexel UniversityNigel W. Fraser - University of PennsylvaniaLisa Faber - Drexel UniversityTimothy M. Block - Drexel University
- Publication Details
- Journal of neurovirology, v 18(3)
- Publisher
- Springer Nature
- Number of pages
- 13
- Grant note
- P01NS033768 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) NS 33768 / National Institute of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000304557500011
- Scopus ID
- 2-s2.0-84863652940
- Other Identifier
- 991019167331904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences
- Virology