Journal article
Direct, real-time measurement of shear stress-induced nitric oxide produced from endothelial cells in vitro
Nitric oxide, v 23(4), pp 335-342
2010
PMID: 20719252
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Nitric oxide (NO) produced by the endothelium is involved in the regulation of vascular tone. Decreased NO production or availability has been linked to endothelial dysfunction in hypercholesterolemia and hypertension. Shear stress-induced NO release is a well-established phenomenon, yet the cellular mechanisms of this response are not completely understood. Experimental limitations have hindered direct, real-time measurements of NO under flow conditions. We have overcome these challenges with a new design for a parallel-plate flow chamber. The chamber consists of two compartments, separated by a Transwell® membrane, which isolates a NO recording electrode located in the upper compartment from flow effects. Endothelial cells are grown on the bottom of the membrane, which is inserted into the chamber flush with the upper plate. We demonstrate for the first time direct real-time NO measurements from endothelial cells with controlled variations in shear stress. Step changes in shear stress from 0.1
dyn/cm
2 to 6, 10, or 20
dyn/cm
2 elicited a transient decrease in NO followed by an increase to a new steady state. An analysis of NO transport suggests that the initial decrease is due to the increased removal rate by convection as flow increases. Furthermore, the rate at which the NO concentration approaches the new steady state is related to the time-dependent cellular response rather than transport limitations of the measurement configuration. Our design offers a method for studying the kinetics of the signaling mechanisms linking NO production with shear stress as well as pathological conditions involving changes in NO production or availability.
Metrics
Details
- Title
- Direct, real-time measurement of shear stress-induced nitric oxide produced from endothelial cells in vitro
- Creators
- Allison M AndrewsDov JaronDonald G BuerkPatrick L KirbyKenneth A Barbee
- Publication Details
- Nitric oxide, v 23(4), pp 335-342
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; [Retired Faculty]
- Web of Science ID
- WOS:000283894500013
- Scopus ID
- 2-s2.0-77958152628
- Other Identifier
- 991014877694304721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology