Journal article
Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1
The EMBO journal, v 15(20), pp 5595-5605
15 Oct 1996
PMID: 8896453
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Abstract
Using the two-hybrid method, we isolated a Saccharomyces cerevisiae cDNA encoding a protein homologous to Schizosaccharomyces pombe protein Dis3sp, using as bait, human GTPase Ran. The DIS3 gene is essential for viability and complements S.pombe mutant dis3-54 which is defective in mitosis. Although Dis3sc has no homology to RanBP1, it bound directly to Ran and the S.cerevisiae Ran homologue Cnr1, but not to the S.cerevisiae RCC1 homologue Srm1. Upon binding to Ran with a 1:1 molar ratio, Dis3sc enhanced a nucleotide-releasing activity of RCC1 on Ran. In the presence of Dis3sc, the K(m) of RCC1 on Ran decreased by half, while the kcat was unchanged. In vivo, Dis3sp was present as oligomers of M(r) 670-200 kDa as previously reported, and the 200 kDa oligomer of Dis3sp was found to include Spi1 and Pim1, the S.pombe homologues of Ran and RCC1, respectively. Although the biological function of the heterotrimeric oligomer consisting of Dis3, Spi1 and Pim1 is unknown, our results indicate that Dis3 is a component of the RCC1-Ran pathway.
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Details
- Title
- Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1
- Creators
- E Noguchi - Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, JapanN Hayashi - Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, JapanY Azuma - Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, JapanT Seki - Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, JapanM Nakamura - Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, JapanN Nakashima - Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, JapanM Yanagida - Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, JapanX He - Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, JapanU Mueller - Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, JapanS Sazer - Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, JapanT Nishimoto - Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan
- Publication Details
- The EMBO journal, v 15(20), pp 5595-5605
- Publisher
- Wiley
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:A1996VQ07100012
- Scopus ID
- 2-s2.0-10544256607
- Other Identifier
- 991014878206004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology