Journal article
Distinct Malaria Parasite Sporozoites Reveal Transcriptional Changes That Cause Differential Tissue Infection Competence in the Mosquito Vector and Mammalian Host
Molecular and cellular biology, v 28(20), pp 6196-6207
15 Oct 2008
PMID: 18710954
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
ABSTRACT The malaria parasite sporozoite transmission stage develops and differentiates within parasite oocysts on the Anopheles mosquito midgut. Successful inoculation of the parasite into a mammalian host is critically dependent on the sporozoite's ability to first infect the mosquito salivary glands. Remarkable changes in tissue infection competence are observed as the sporozoites transit from the midgut oocysts to the salivary glands. Our microarray analysis shows that compared to oocyst sporozoites, salivary gland sporozoites upregulate expression of at least 124 unique genes. Conversely, oocyst sporozoites show upregulation of at least 47 genes (upregulated in oocyst sporozoites [UOS genes]) before they infect the salivary glands. Targeted gene deletion of UOS3, encoding a putative transmembrane protein with a thrombospondin repeat that localizes to the sporozoite secretory organelles, rendered oocyst sporozoites unable to infect the mosquito salivary glands but maintained the parasites' liver infection competence. This phenotype demonstrates the significance of differential UOS expression. Thus, the UIS-UOS gene classification provides a framework to elucidate the infectivity and transmission success of Plasmodium sporozoites on a whole-genome scale. Genes identified herein might represent targets for vector-based transmission blocking strategies (UOS genes), as well as strategies that prevent mammalian host infection (UIS genes).
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Details
- Title
- Distinct Malaria Parasite Sporozoites Reveal Transcriptional Changes That Cause Differential Tissue Infection Competence in the Mosquito Vector and Mammalian Host
- Creators
- Sebastian A Mikolajczak - Seattle Biomedical Research Institute, Seattle, Washington 98109Hilda Silva-Rivera - Seattle Biomedical Research Institute, Seattle, Washington 98109Xinxia Peng - Seattle Biomedical Research Institute, Seattle, Washington 98109Alice S Tarun - Seattle Biomedical Research Institute, Seattle, Washington 98109Nelly Camargo - Seattle Biomedical Research Institute, Seattle, Washington 98109Vanessa Jacobs-Lorena - Seattle Biomedical Research Institute, Seattle, Washington 98109Thomas M Daly - Division of Molecular Parasitology, Department of Microbiology & Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129Lawrence W Bergman - Division of Molecular Parasitology, Department of Microbiology & Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129Patricia de la Vega - Walter Reed Army Institute for Research, Silver Spring, Maryland 20910Jack Williams - Walter Reed Army Institute for Research, Silver Spring, Maryland 20910Ahmed S. I Aly - Seattle Biomedical Research Institute, Seattle, Washington 98109Stefan H. I Kappe - Seattle Biomedical Research Institute, Seattle, Washington 98109, Department of Global Health, University of Washington, Seattle, Washington 98195
- Publication Details
- Molecular and cellular biology, v 28(20), pp 6196-6207
- Publisher
- American Society for Microbiology (ASM)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; [Retired Faculty]
- Web of Science ID
- WOS:000259634800005
- Scopus ID
- 2-s2.0-50849133642
- Other Identifier
- 991014877718304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology