Journal article
Distribution of type I Fc sub( epsilon )-receptors on the surface of mast cells probed by fluorescence resonance energy transfer
Biophysical journal, Vol.64(1), pp.110-120
01 Jan 1993
Abstract
The aggregation state of type I Fc sub( epsilon )-receptors (Fc sub( epsilon )RI) on the surface of single living mast cells was investigated by resonance fluorescence energy transfer. The efficiency of energy transfer between the ligands carrying distinct fluorophores was determined on single cells in a microscope by analyzing the photobleaching kinetics of the donor fluorophore in the presence and absence of receptor ligands labeled with acceptor fluorophores. To rationalize the energy transfer data, we developed a theoretical model describing the dependence of the energy transfer efficiency on the geometry of the fluorescently labeled macromolecular ligands and their aggregation state on the cell surface. Comparison of the theoretical modeling results with the experimental energy transfer data clearly suggests that the Fc sub( epsilon )RI are monovalent, randomly distributed plasma membrane proteins. The method provides a novel approach for determining the aggregation state of cell surface components.
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Details
- Title
- Distribution of type I Fc sub( epsilon )-receptors on the surface of mast cells probed by fluorescence resonance energy transfer
- Creators
- U KubitscheckR Schweitzer-StennerD Arndt-JovinT JovinI Pecht
- Publication Details
- Biophysical journal, Vol.64(1), pp.110-120
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Identifiers
- 991019196449804721