Journal article
Dopamine D-1 receptor-mediated enhancement of NMDA receptor trafficking requires rapid PKC-dependent synaptic insertion in the prefrontal neurons
Journal of neurochemistry, v 114(1), pp 62-73
01 Jul 2010
PMID: 20374423
Abstract
P>Understanding the interaction between dopamine and glutamate, particularly the interaction of dopamine and NMDA receptors, may enable a more rational approach to the treatment of schizophrenia, drug addiction, and other psychiatric disorders. We show that, in prefrontal cortical neurons, dopamine D-1-induced enhancement of NMDA receptor function depends on rapid insertion of new NMDA receptor 2B subunits on the synaptic surface. Protein kinase A (PKA) inhibitor, but not protein kinase C (PKC) inhibitor, completely blocked dopamine D-1 agonist SKF-81297-induced increase of the total expression of NMDA receptors. Furthermore, SKF-81297 failed to alter the surface expression and synaptic insertion of NMDA receptors in the presence of PKA inhibitor, phospholipase C inhibitor, PKC inhibitor, or Src family kinase inhibitor. Our data suggest that D-1-mediated enhancement of NMDA current depends on the NMDA receptor trafficking through rapid synaptic insertion and both PKA and PKC signaling pathways play important roles in the regulatory process. Although both PKA and PKC mediate the D-1-induced enhancement of NMDA receptors, the phospholipase C-PKC-Src pathway is only required for surface expression and new synaptic insertion of NMDA receptors.
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Details
- Title
- Dopamine D-1 receptor-mediated enhancement of NMDA receptor trafficking requires rapid PKC-dependent synaptic insertion in the prefrontal neurons
- Creators
- Yan-Chun Li - Drexel University, Neurobiology and AnatomyGang Liu - College Station Medical CenterJian-Li Hu - Drexel Univ, Coll Med, Dept Neurobiol & Anat, Philadelphia, PA 19129 USAWen-Jun Gao - Drexel University, Neurobiology and AnatomyYue-Qiao Huang - Drexel Univ, Coll Med, Dept Neurobiol & Anat, Philadelphia, PA 19129 USA
- Publication Details
- Journal of neurochemistry, v 114(1), pp 62-73
- Publisher
- Wiley
- Number of pages
- 12
- Grant note
- R01MH085666 / NATIONAL INSTITUTE OF MENTAL HEALTH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH) Drexel University College of Medicine NARSAD R21MH232307; R01MH232395 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000278567100006
- Other Identifier
- 991014877916404721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences