Files and links (1)
Accepted (AM)Open Access (License Unspecified), Open
Journal article
Dopamine Increases CD14(+)CD16(+) Monocyte Transmigration across the Blood Brain Barrier: Implications for Substance Abuse and HIV Neuropathogenesis
Journal of neuroimmune pharmacology, v 12(2), pp 353-370
01 Jun 2017
PMCID: PMC5406247
PMID: 28133717
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In human immunodeficiency virus-1 (HIV) infected individuals, substance abuse may accelerate the development and/or increase the severity of HIV associated neurocognitive disorders (HAND). It is proposed that CD14(+)CD16(+) monocytes mediate HIV entry into the central nervous system (CNS) and that uninfected and infected CD14(+)CD16(+) monocyte transmigration across the blood brain barrier (BBB) contributes to the establishment and propagation of CNS HIV viral reservoirs and chronic neuroinflammation, important factors in the development of HAND. The effects of substance abuse on the frequency of CD14(+)CD16(+) monocytes in the peripheral circulation and on the entry of these cells into the CNS during HIV neuropathogenesis are not known. PBMC from HIV infected individuals were analyzed by flow cytometry and we demonstrate that the frequency of peripheral blood CD14(+)CD16(+) monocytes in HIV infected substance abusers is increased when compared to those without active substance use. Since drug use elevates extracellular dopamine concentrations in the CNS, we examined the effects of dopamine on CD14(+)CD16(+) monocyte transmigration across our in vitro model of the human BBB. The transmigration of this monocyte subpopulation is increased by dopamine and the dopamine receptor agonist, SKF 38393, implicating D1-like dopamine receptors in the increase in transmigration elicited by this neurotransmitter. Thus, elevated extracellular CNS dopamine may be a novel common mechanism by which active substance use increases uninfected and HIV infected CD14(+)CD16(+) monocyte transmigration across the BBB. The influx of these cells into the CNS may increase viral seeding and neuroinflammation, contributing to the development of HIV associated neurocognitive impairments.
Metrics
Details
- Title
- Dopamine Increases CD14(+)CD16(+) Monocyte Transmigration across the Blood Brain Barrier: Implications for Substance Abuse and HIV Neuropathogenesis
- Creators
- Tina M. Calderon - Yeshiva UniversityDionna W. Williams - Yeshiva UniversityLillie Lopez - Yeshiva UniversityEliseo A. Eugenin - Rutgers, The State University of New JerseyLaura Cheney - Yeshiva UniversityPeter J. Gaskill - Yeshiva UniversityMike Veenstra - Yeshiva UniversityKathryn Anastos - Yeshiva UniversitySusan Morgello - Icahn School of Medicine at Mount SinaiJoan W. Berman - Yeshiva University
- Publication Details
- Journal of neuroimmune pharmacology, v 12(2), pp 353-370
- Publisher
- Springer Nature
- Number of pages
- 18
- Grant note
- DA025567; MH075679; MH090958; DA029476; MH096625; Al035004; Al142590; MH083501; MH100931 / U.S. NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA K99DA044838 / NATIONAL INSTITUTE ON DRUG ABUSE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Drug Abuse (NIDA); European Commission United Negro College Fund/Merck Graduate Science Dissertation Fellowship U01AI035004 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) MH080663 / Mount Sinai Institute for NeuroAIDS Disparities Pilot Funds CFAR/AI051519 / Center for AIDS Research at the Albert Einstein College of Medicine, Montefiore Medical Center R25MH080663 / NATIONAL INSTITUTE OF MENTAL HEALTH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine; Pharmacology and Physiology; Drexel University
- Web of Science ID
- WOS:000400077600013
- Scopus ID
- 2-s2.0-85010971976
- Other Identifier
- 991020099141804721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences
- Pharmacology & Pharmacy