Journal article
Dopamine Receptor Stimulation Decreases Cytosolic γ Protein Kinase C Immunoreactivity in Rat Hippocampal Slices: Evidence for Increased Ca2+‐Dependent Proteolysis
Journal of neurochemistry, v 65(4), pp 1622-1630
Oct 1995
PMID: 7561857
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Abstract
: The effect of dopamine (DA) receptor stimulation on the distribution of γ protein kinase C (γPKC) in hippocampal slices was assessed. Nanomolar concentrations of DA decreased cytosolic γPKC (56%) without altering membrane γPKC levels, resulting in decreased total γPKC immunoreactivity. The maximal decrease in cytosolic γPKC occurred at 20 min of incubation and was significantly blocked by the D1 DA antagonist SCH 23390 (10−6M) but not by the D2 antagonist sulpiride (10−5M). The D1 agonists SKF 38393 and A 77636 mimicked the effect of DA with similar responses produced at 10 µM and 1 nM, respectively. The D2 agonist quinpirole had no effect on γPKC immunoreactivity, thus indicating that this dopaminergic response is mediated through a D1‐like receptor. DA had no effect on α, δ, or ζPKC isozyme immunoreactivity in the same hippocampal preparations. The DA‐induced decrease in cytosolic γPKC immunoreactivity was blocked by the Ca2+‐dependent protease inhibitor N‐acetyl‐Leu‐Leu‐norleucinal (100 µM) and by the inorganic Ca2+ channel blocker Co2+. The data suggest that DA stimulates a D1‐like DA receptor, which increases the influx of Ca2+ and activates the Ca2+‐dependent proteolysis of γPKC.
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Details
- Title
- Dopamine Receptor Stimulation Decreases Cytosolic γ Protein Kinase C Immunoreactivity in Rat Hippocampal Slices: Evidence for Increased Ca2+‐Dependent Proteolysis
- Creators
- Karin A. Yurko‐MauroEitan Friedman - Woman's Medical College of Pennsylvania
- Publication Details
- Journal of neurochemistry, v 65(4), pp 1622-1630
- Publisher
- Blackwell Science Ltd
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1995RW33500022
- Other Identifier
- 991019183972804721
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- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences