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Journal article
Dopamine increases in cat carotid body during excitation by carbon monoxide: Implications for a chromophore theory of chemoreception
Journal of the autonomic nervous system, v 67(3)
1997
PMID: 9479664
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Studies of dopamine (DA) release were conducted with 10 perfused/superfused cat carotid bodies using shallow recessed Nafion polymer-coated microsensors (tips ∼5
μm). Simultaneous measurements of tissue DA and neuronal discharge (ND) from the sinus nerve were made after switching from normoxic, normocapnic control perfusate (20% O
2, 5% CO
2, balance N
2) to a normoxic, normocapnic perfusate equilibrated with a high tension (>550 Torr) of carbon monoxide (CO). When high
P
CO perfusate was delivered in the dark, ND increased from a baseline of 89±24 (SE) impulses/s, to a peak excitation of 374±44 impulses/s within 15–30 s. Excitation then diminished to a plateau of 281±36 impulses/s within 1–2 min. Both peak and plateau ND were significantly above baseline (
P<0.05). Average tissue DA values increased above basal levels by +7.2±1.0 and +5.6±0.6
μM, respectively, during the peak and plateau ND phases (
P<0.05). Bright light restored the chemosensory activity to baseline, but had no effect on DA. Both chemosensory excitation and tissue DA responses to high CO in the dark were diminished in 3 carotid bodies perfused with Ca
2+-free solutions. Responses were reduced even further with Ca
2+ chelator (EGTA) in the perfusate. The results suggest that the effect of high
P
CO on DA release and chemosensory excitation are dependent on Ca
2+ in the media, but the two events are not coupled.
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Details
- Title
- Dopamine increases in cat carotid body during excitation by carbon monoxide: Implications for a chromophore theory of chemoreception
- Creators
- D.G Buerk - University of PennsylvaniaD.K Chugh - University of PennsylvaniaS Osanai - University of PennsylvaniaA Mokashi - University of PennsylvaniaS Lahiri - University of Pennsylvania
- Publication Details
- Journal of the autonomic nervous system, v 67(3)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000071393600003
- Scopus ID
- 2-s2.0-0031565139
- Other Identifier
- 991019231627704721
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- Web of Science research areas
- Neurosciences