Journal article
Drastically Lowered Protein Adsorption on Microbicidal Hydrophobic/Hydrophilic Polyelectrolyte Multilayers
Biomacromolecules, v 13(3), pp 719-726
01 Mar 2012
PMID: 22300304
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Polyelectrolyte multilayer films assembled from a hydrophobic N-alkylated polyethylenimine and a hydrophilic polyacrylate were discovered to exhibit strong antifouling, as well as antimicrobial, activities. Surfaces coated with these layer-by-layer (LbL) films, which range from 6 to 10 bilayers (up to 45 nm in thickness), adsorbed up to 20 times less protein from blood plasma than the uncoated controls. The dependence of the antifouling activity on the nature of the polycation, as well as on assembly conditions and the number of layers in the LbL films, was investigated. Changing the hydrophobicity of the polycation altered the surface composition and the resistance to protein adsorption of the LbL films. Importantly, this resistance was greater for coated surfaces with the polyanion on top; for these films, the average zeta potential pointed to a near neutral surface charge, thus, presumably minimizing their electrostatic interactions with the protein. The film surface exhibited a large contact angle hysteresis, indicating a heterogeneous topology likely due to the existence of hydrophobic hydrophilic regions on the surface. Scanning electron micrographs of the film surface revealed the existence of nanoscale domains. We hypothesize that the existence of hydrophobic/hydrophilic nanodomains, as well as surface charge neutrality, contributes to the LbL film's resistance to protein adsorption.
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Details
- Title
- Drastically Lowered Protein Adsorption on Microbicidal Hydrophobic/Hydrophilic Polyelectrolyte Multilayers
- Creators
- Sze Yinn Wong - Massachusetts Institute of TechnologyLin Han - MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USAKsenia Timachova - Massachusetts Institute of TechnologyJovana Veselinovic - Massachusetts Institute of TechnologyMd Nasirn Hyder - MIT, Dept Chem Engn, Cambridge, MA 02139 USAChristine Ortiz - Massachusetts Institute of TechnologyAlexander M. Klibanov - Massachusetts Institute of TechnologyPaula T. Hammond - Massachusetts Institute of Technology
- Publication Details
- Biomacromolecules, v 13(3), pp 719-726
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 8
- Grant note
- U.S. Army through the Institute for Soldier Nanotechnologies (ISN) at the Massachusetts Institute of Technology (MIT)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000301318100018
- Scopus ID
- 2-s2.0-84863856459
- Other Identifier
- 991019176795204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Organic
- Polymer Science