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Dual-opposite injection capillary electrophoresis: Principles and misconceptions
Journal article   Peer reviewed

Dual-opposite injection capillary electrophoresis: Principles and misconceptions

Donna M Blackney and Joe P Foley
Electrophoresis, v 38(5), pp 607-616
Mar 2017
PMID: 27859385

Abstract

Models, Theoretical Cations - isolation & purification Milk - chemistry Electrophoresis, Capillary Animals Cations - chemistry Anions - analysis Cations - analysis Anions - chemistry Anions - isolation & purification Rain - chemistry
Dual-opposite injection capillary electrophoresis (DOI-CE) is a separation technique that utilizes both ends of the capillary for sample introduction. The electroosmotic flow (EOF) is suppressed to allow all ions to reach the detector quickly. Depending on the individual electrophoretic mobilities of the analytes of interest and the effective length that each analyte travels to the detection window, the elution order of analytes in a DOI-CE separation can vary widely. This review discusses the principles, applications, and limitations of dual-opposite injection capillary electrophoresis. Common misconceptions regarding DOI-CE are clarified.

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Web of Science research areas
Biochemical Research Methods
Chemistry, Analytical
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