Journal article
Dual opposite injection electrokinetic chromatography: nonionic microemulsion pseudostationary phase and novel approach to electrokinetic sampling bias
Electrophoresis, v 25(4-5), pp 653-663
Feb 2004
PMID: 14981693
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Dual opposite injection capillary electrophoresis (DOI-CE) is a family of CE techniques in which the sample is introduced into both ends of the capillary. For the analysis of compounds with widely varying pKa values using a voltage-driven separation scheme, DOI-CE is superior to conventional CE with sample introduction at only one end of the capillary due to DOI-CE's broader elution window. To enhance the DOI-CE separation, a running buffer with a microemulsion system was developed. Since DOI-CE works best under conditions of low electroosmotic flow (EOF), the suppression of EOF via the addition of a multiply charged cation (e.g., Zn2+) to the buffer was investigated, and was found to suppress the EOF effectively at moderate concentrations (2.5-10 mM). Three different dual opposite injection modes were studied: simultaneous electrokinetic injection, sequential electrokinetic injection, and sequential hydrodynamic injection. The injection bias in the first two electrokinetic injection modes was compared with the sequential hydrodynamic injection. Corrections in the bias of the electrokinetic injections were discussed, and an improved approach was suggested. Finally, the effect of the relative concentration of the multiply charged cation in the sample plug and running buffer on the peak shape of co-electroosmotic and counter-electroosmotic ions was examined, and found to be much more influential on the latter.
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Details
- Title
- Dual opposite injection electrokinetic chromatography: nonionic microemulsion pseudostationary phase and novel approach to electrokinetic sampling bias
- Creators
- Marilyn X Zhou - Department of Chemistry, Drexel University, Philadelphia, PA 19104, USAJoe P Foley
- Publication Details
- Electrophoresis, v 25(4-5), pp 653-663
- Publisher
- Wiley; Germany
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000220046200015
- Scopus ID
- 2-s2.0-1542375243
- Other Identifier
- 991014877860604721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical