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Journal article
Dynamic Loading and Tendon Healing Affect Multiscale Tendon Properties and ECM Stress Transmission
Scientific reports, v 8(Jul (E-published)), pp 10854-13
18 Jul 2018
PMID: 30022076
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The extracellular matrix (ECM) is the primary biomechanical environment that interacts with tendon cells (tenocytes). Stresses applied via muscle contraction during skeletal movement transfer across structural hierarchies to the tenocyte nucleus in native uninjured tendons. Alterations to ECM structural and mechanical properties due to mechanical loading and tissue healing may affect this multiscale strain transfer and stress transmission through the ECM. This study explores the interface between dynamic loading and tendon healing across multiple length scales using living tendon explants. Results show that macroscale mechanical and structural properties are inferior following high magnitude dynamic loading (fatigue) in uninjured living tendon and that these effects propagate to the microscale. Although similar macroscale mechanical effects of dynamic loading are present in healing tendon compared to uninjured tendon, the microscale properties differed greatly during early healing. Regression analysis identified several variables (collagen and nuclear disorganization, cellularity, and F-actin) that directly predict nuclear deformation under loading. Finite element modeling predicted deficits in ECM stress transmission following fatigue loading and during healing. Together, this work identifies the multiscale response of tendon to dynamic loading and healing, and provides new insight into microenvironmental features that tenocytes may experience following injury and after cell delivery therapies.
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Details
- Title
- Dynamic Loading and Tendon Healing Affect Multiscale Tendon Properties and ECM Stress Transmission
- Creators
- Benjamin R. Freedman - University of PennsylvaniaAshley B. Rodriguez - University of PennsylvaniaRyan J. Leiphart - University of PennsylvaniaJoseph B. Newton - University of PennsylvaniaEhsan Ban - University of PennsylvaniaJoseph J. Sarver - Drexel UniversityRobert L. Mauck - University of PennsylvaniaVivek B. Shenoy - Department of Materials Science and Engineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, USALouis J. Soslowsky - University of Pennsylvania
- Publication Details
- Scientific reports, v 8(Jul (E-published)), pp 10854-13
- Publisher
- Springer Nature
- Number of pages
- 13
- Grant note
- NSF GRFP; National Science Foundation (NSF); NSF - Office of the Director (OD) T32AR007132 / NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) P30AR050950; T32AR007132 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000439026000015
- Scopus ID
- 2-s2.0-85050340612
- Other Identifier
- 991019169367404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biophysics