Files and links (1)
Published, Version of Record (VoR)CC BY-NC-SA V4.0, Open
Journal article
Dynamic mechanical properties of the tissue-engineered matrix associated with individual chondrocytes
Journal of biomechanics, v 43(3), pp 469-476
2010
PMCID: PMC2813355
PMID: 19889416
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The success of cell-based tissue engineering approaches in restoring biological function will be facilitated by a comprehensive fundamental knowledge of the temporal evolution of the structure and properties of the newly synthesized matrix. Here, we quantify the dynamic oscillatory mechanical behavior of the engineered matrix associated with individual chondrocytes cultured
in vitro for up to 28 days in alginate scaffolds. The magnitude of the complex modulus (|
E*|) and phase shift (
δ) were measured in culture medium using Atomic Force Microscopy (AFM)-based nanoindentation in response to an imposed oscillatory deformation (amplitude ∼5
nm) as a function of frequency (
f=1–316
Hz), probe tip geometry (2.5
μm radius sphere and 50
nm radius square pyramid), and in the absence and presence of growth factors (GF, insulin growth factor-1, IGF-1, and osteogenic protein-1, OP-1). |
E*| for all conditions increased nonlinearly with frequency dependence approximately
f
1/2 and ranged between ∼1 and 25
kPa. This result, along with theoretical calculations of the characteristic poroelastic relaxation frequency,
f
p
, (∼50–90
Hz) suggested that this time-dependent behavior was governed primarily by fluid flow-dependent poroelasticity, rather than flow-independent viscoelastic processes associated with the solid matrix. |
E*(
f)| increased, (
f) decreased, and the hydraulic permeability,
k, decreased with time in culture and with growth factor treatment. This trend of a more elastic-like response was thought to be associated with increased macromolecular biosynthesis, density, and a more mature matrix structure/organization.
Metrics
Details
- Title
- Dynamic mechanical properties of the tissue-engineered matrix associated with individual chondrocytes
- Creators
- BoBae Lee - Massachusetts Institute of TechnologyLin Han - Massachusetts Institute of TechnologyEliot H. Frank - Massachusetts Institute of TechnologySusan Chubinskaya - Rush University Medical CenterChristine Ortiz - Massachusetts Institute of TechnologyAlan J. Grodzinsky - Massachusetts Institute of Technology
- Publication Details
- Journal of biomechanics, v 43(3), pp 469-476
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000274927800012
- Scopus ID
- 2-s2.0-74449087128
- Other Identifier
- 991019186969004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biophysics
- Engineering, Biomedical