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Dynamic mechanical properties of the tissue-engineered matrix associated with individual chondrocytes
Journal article   Open access   Peer reviewed

Dynamic mechanical properties of the tissue-engineered matrix associated with individual chondrocytes

BoBae Lee, Lin Han, Eliot H. Frank, Susan Chubinskaya, Christine Ortiz and Alan J. Grodzinsky
Journal of biomechanics, v 43(3), pp 469-476
2010
DOI: https://doi.org/10.1016/j.jbiomech.2009.09.053
PMID: 19889416
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1016/j.jbiomech.2009.09.053View
Published, Version of Record (VoR)CC BY-NC-SA V4.0,  Open

Abstract

AFM Chondrocytes Dynamic compression Growth factors Nanomechanics Pericellular matrix Tissue engineering
The success of cell-based tissue engineering approaches in restoring biological function will be facilitated by a comprehensive fundamental knowledge of the temporal evolution of the structure and properties of the newly synthesized matrix. Here, we quantify the dynamic oscillatory mechanical behavior of the engineered matrix associated with individual chondrocytes cultured in vitro for up to 28 days in alginate scaffolds. The magnitude of the complex modulus (| E*|) and phase shift ( δ) were measured in culture medium using Atomic Force Microscopy (AFM)-based nanoindentation in response to an imposed oscillatory deformation (amplitude ∼5 nm) as a function of frequency ( f=1–316 Hz), probe tip geometry (2.5 μm radius sphere and 50 nm radius square pyramid), and in the absence and presence of growth factors (GF, insulin growth factor-1, IGF-1, and osteogenic protein-1, OP-1). | E*| for all conditions increased nonlinearly with frequency dependence approximately f 1/2 and ranged between ∼1 and 25 kPa. This result, along with theoretical calculations of the characteristic poroelastic relaxation frequency, f p , (∼50–90 Hz) suggested that this time-dependent behavior was governed primarily by fluid flow-dependent poroelasticity, rather than flow-independent viscoelastic processes associated with the solid matrix. | E*( f)| increased, ( f) decreased, and the hydraulic permeability, k, decreased with time in culture and with growth factor treatment. This trend of a more elastic-like response was thought to be associated with increased macromolecular biosynthesis, density, and a more mature matrix structure/organization.

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Collaboration types
Domestic collaboration
Web of Science research areas
Biophysics
Engineering, Biomedical
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