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Journal article
Dynamic nanomechanics of individual bone marrow stromal cells and cell-matrix composites during chondrogenic differentiation
Journal of biomechanics, v 48(1), pp 171-175
02 Jan 2015
PMID: 25468666
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Dynamic nanomechanical properties of bovine bone marrow stromal cells (BMSCs) and their newly synthesized cartilage-like matrices were studied at nanometer scale deformation amplitudes. The increase in their dynamic modulus, |E*| (e.g., 2.4 +/- 0.4 kPa at 1 Hz to 9.7 +/- 0.2 kPa at 316 Hz at day 21, mean +/- SEM), and phase angle, delta, (e.g., 15 +/- 2 degrees at 1 Hz to 74 +/- 1 degrees at 316 Hz at day 21) with increasing frequency were attributed to the fluid flow induced poroelasticity, governed by both the newly synthesized matrix and the intracellular structures. The absence of culture duration dependence suggested that chondrogenesis of BMSCs had not yet resulted in the formation of a well-organized matrix with a hierarchical structure similar to cartilage. BMSC-matrix composites demonstrated different poro-viscoelastic frequency-dependent mechanical behavior and energy dissipation compared to chondrocyte-matrix composites due to differences in matrix molecular constituents, structure and cell properties. This study provides important insights into the design of optimal protocols for tissue-engineered cartilage products using chondrocytes and BMSCs. (C) 2014 Elsevier Ltd. All rights reserved.
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Details
- Title
- Dynamic nanomechanics of individual bone marrow stromal cells and cell-matrix composites during chondrogenic differentiation
- Creators
- Bobae Lee - Massachusetts Institute of TechnologyLin Han - Drexel Univ, Sch Biomed Engn Sci & Hlth Syst, Philadelphia, PA 19104 USAEliot H. Frank - Massachusetts Institute of TechnologyAlan J. Grodzinsky - Massachusetts Institute of TechnologyChristine Ortiz - Massachusetts Institute of Technology
- Publication Details
- Journal of biomechanics, v 48(1), pp 171-175
- Publisher
- Elsevier
- Number of pages
- 5
- Grant note
- Institute for Soldier Nanotechnologies at MIT through the U.S. Army Research Office CMMI-0758651 / National Science Foundation; National Science Foundation (NSF) Drexel University N00244-09-1-0064 / National Security Science and Engineering Faculty Fellowship R01AR060331 / NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) AR060331 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000348336200024
- Scopus ID
- 2-s2.0-84921988400
- Other Identifier
- 991019168774704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biophysics
- Engineering, Biomedical