Journal article
Dynamic tissue engineering scaffolds with stimuli-responsive macroporosity formation
Biomaterials, v 34(17), pp 4251-4258
01 Jun 2013
PMID: 23489920
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Macropores in tissue engineering scaffolds provide space for vascularization, cell-proliferation and cellular interactions, and is crucial for successful tissue regeneration. Modulating the size and density of macropores may promote desirable cellular processes at different stages of tissue development. Most current techniques for fabricating macroporous scaffolds produce fixed macroporosity and do not allow the control of porosity during cell culture. Most macropore-forming techniques also involve non-physiological conditions, such that cells can only be seeded in a post-fabrication process, which often leads to low cell seeding efficiency and uneven cell distribution. Here we report a process to create dynamic hydrogels as tissue engineering scaffolds with tunable macroporosity using stimuli-responsive porogens of gelatin, alginate and hyaluronic acid, which degrade in response to specific stimuli including temperature, chelating and enzymatic digestion, respectively. SEM imaging confirmed sequential pore formation in response to sequential stimulations: 37 degrees C on day 0, EDTA on day 7, and hyaluronidase on day 14. Bovine chondrocytes were encapsulated in the Alg porogen, which served as cell-delivery vehicles, and changes in cell viability, proliferation and tissue formation during sequential stimuli treatments were evaluated. Our results showed effective cell release from Alg porogen with high cell viability and markedly increased cell proliferation and spreading throughout the 3D hydrogels. Dynamic pore formation also led to significantly enhanced type II and X collagen production by chondrocytes. This platform provides a valuable tool to create stimuli-responsive scaffolds with dynamic macroporosity for a broad range of tissue engineering applications, and may also be used for fundamental studies to examine cell responses to dynamic niche properties. (C) 2013 Elsevier Ltd. All rights reserved.
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Details
- Title
- Dynamic tissue engineering scaffolds with stimuli-responsive macroporosity formation
- Creators
- Li-Hsin Han - Stanford UniversityJanice H. Lai - Stanford UniversityStephanie Yu - Stanford UniversityFan Yang - Stanford University
- Publication Details
- Biomaterials, v 34(17), pp 4251-4258
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- March of Dimes Foundation; March of Dimes Stanford Bio-X Interdisciplinary Initiative grant McCormick Faculty Award
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics; Drexel University
- Web of Science ID
- WOS:000317700400006
- Scopus ID
- 2-s2.0-84875531648
- Other Identifier
- 991020100050204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials