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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Dynorphin-containing axons directly innervate noradrenergic neurons in the rat nucleus locus coeruleus
Neuroscience, v 145(3), pp 1077-1086
30 Mar 2007
PMID: 17289275
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Stress causes increased dynorphin (DYN) expression in limbic brain regions and antagonism of kappa-opioid receptors may offer therapeutic potential for the treatment of depression. A potential site of DYN action relevant to stress and related neuropsychiatric disorders is the locus coeruleus (LC), the primary source of forebrain norepinephrine. Therefore, using immunofluorescence and immunoelectron microscopic analyses, we characterized the cellular substrates for interactions between DYN and tyrosine hydroxylase (TH), a catecholamine synthesizing enzyme in single sections through the rat LC. Light microscopic analysis of DYN immunoreactivity indicated that DYN fibers are distributed within the core and pericoerulear subregions of the LC. Using electron microscopy, immunoperoxidase labeling for DYN was primarily found in axon terminals, although in some cases was diffusely localized to somatodendritic processes. When DYN-containing axons formed synaptic contacts, they typically (89%) exhibited an asymmetric morphology. Almost a third (28%) of the postsynaptic targets of DYN-containing axons contained immunogold labeling for TH. These findings reveal some diversity as to the localization of DYN in the LC within axons that contact both TH and non-TH containing dendrites. However, the present data provide the first ultrastructural evidence that DYN-containing axon terminals directly innervate catecholaminergic LC dendrites. Moreover, DYN axon terminals targeting catecholaminergic LC dendrites via asymmetric synapses are consistent with localization within excitatory type afferents to the LC. Therefore, direct modulation of catacholaminergic LC neurons maybe an important site of action for DYN relevant to stress and stress-related disorders.
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Details
- Title
- Dynorphin-containing axons directly innervate noradrenergic neurons in the rat nucleus locus coeruleus
- Creators
- B.A.S. Reyes - Jefferson Hospital for NeuroscienceA.D. Johnson - Thomas Jefferson UniversityJ.D. Glaser - Thomas Jefferson UniversityK.G. Commons - Harvard Medical SchoolE.J. Van Bockstaele - Thomas Jefferson University
- Publication Details
- Neuroscience, v 145(3), pp 1077-1086
- Publisher
- Elsevier
- Number of pages
- 10
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000245516000027
- Scopus ID
- 2-s2.0-33947398024
- Other Identifier
- 991021903413004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences