Journal article
EFFECT OF NUTRIENT AVAILABILITY ON THE BIOCHEMICAL AND ELEMENTAL STOICHIOMETRY IN THE FRESHWATER DIATOM STEPHANODISCUS MINUTULUS (BACILLARIOPHYCEAE)
Journal of phycology, v 36(3), pp 510-522
Jun 2000
PMID: 29544004
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The objective of this study was to examine the differences in the biochemical and elemental stoichiometry of a freshwater centric diatom, Stephanodiscus minutulus (Grun.), under various nutrient regimes. Stephanodiscus minutulus was grown at μ
or 22% of μ
under limitation by silicon, nitrogen, or phosphorus. Cell sizes for nutrient-limited cultures were significantly smaller than the non-limited cell sizes, with N-limited cells being significantly smaller than all other treatments. Compared with the nutrient-replete treatment, both carbohydrates and lipids increased in Si- and P-limited cells, whereas carbohydrates increased but proteins decreased in N-limited cells. All of the growth-limited cells showed an increase of carbohydrate and triglyceride, and a decrease of cell size and polar lipids as a percentage of total lipids. The non-limited cells also had a significantly higher chl a concentration and galactolipids as a percentage of total lipids than any of the limited treatments, and the low-Si and low-P cells had significantly higher values than the low-N cells. The particulate C concentrations showed significant differences between treatments, with the Si- and P-limited treatments being significantly higher than the N- and non-limited treatments. Particulate Si did not show a strong relationship with any of the parameters measured, and it was the only parameter with no differences between treatments. The low-Si cells had a significantly higher P content (about two times more) than any other treatment, presumably owing to the luxury consumption of P, and a correspondingly high phospholipid concentration. The elemental data showed that S. minutulus had a high P demand with low optimum N:P (4) and Si:P (10) ratios and a C:N:P ratio of 109:16:2.3. The particulate C showed a positive relationship with POM (r = 0.93), dry weight (r = 0.88), lipid (r = 0.87) and protein (r = 0.84, all P < 0.0001). Particulate N showed a positive relationship with galactolipids (r = 0.95), protein (r = 0.90), dry weight (r = 0.78), lipid (r = 0.75), and cell volume (r = 0.64, all P < 0.0001). It is evident that nutrient limitation in the freshwater diatom S. minutulus has pronounced effects on its biochemical and elemental stoichiometry.
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Details
- Title
- EFFECT OF NUTRIENT AVAILABILITY ON THE BIOCHEMICAL AND ELEMENTAL STOICHIOMETRY IN THE FRESHWATER DIATOM STEPHANODISCUS MINUTULUS (BACILLARIOPHYCEAE)
- Creators
- Scott G Lynn - Department of Bioscience and Biotechnology, Drexel University, Philadelphia, Pennsylvania 19104School of Environmental Science, Engineering and Policy, Drexel University, Philadelphia, Pennsylvania 19104Academy of Natural Sciences Philadelphia, 1900 Benjamin Franklin Parkway, Philadelphia, Pennsylvania 19103School of Environmental Science, Engineering and Policy, Drexel University, Philadelphia, Pennsylvania 19104Susan S Kilham - Department of Bioscience and Biotechnology, Drexel University, Philadelphia, Pennsylvania 19104School of Environmental Science, Engineering and Policy, Drexel University, Philadelphia, Pennsylvania 19104Academy of Natural Sciences Philadelphia, 1900 Benjamin Franklin Parkway, Philadelphia, Pennsylvania 19103School of Environmental Science, Engineering and Policy, Drexel University, Philadelphia, Pennsylvania 19104Daniel A Kreeger - Department of Bioscience and Biotechnology, Drexel University, Philadelphia, Pennsylvania 19104School of Environmental Science, Engineering and Policy, Drexel University, Philadelphia, Pennsylvania 19104Academy of Natural Sciences Philadelphia, 1900 Benjamin Franklin Parkway, Philadelphia, Pennsylvania 19103School of Environmental Science, Engineering and Policy, Drexel University, Philadelphia, Pennsylvania 19104Sebastian J Interlandi - Department of Bioscience and Biotechnology, Drexel University, Philadelphia, Pennsylvania 19104School of Environmental Science, Engineering and Policy, Drexel University, Philadelphia, Pennsylvania 19104Academy of Natural Sciences Philadelphia, 1900 Benjamin Franklin Parkway, Philadelphia, Pennsylvania 19103School of Environmental Science, Engineering and Policy, Drexel University, Philadelphia, Pennsylvania 19104
- Publication Details
- Journal of phycology, v 36(3), pp 510-522
- Publisher
- United States
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biodiversity, Earth, and Environmental Science (BEES)
- Web of Science ID
- WOS:000087890100007
- Scopus ID
- 2-s2.0-0033851422
- Other Identifier
- 991014877831804721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Marine & Freshwater Biology
- Plant Sciences