Journal article
EFFECTS OF ETHIDIUM BROMIDE ON THE CYTOCHROME CONTENT AND ULTRASTRUCTURE OF L CELL MITOCHONDRIA
The Journal of cell biology, v 51(2), pp 514-524
01 Nov 1971
PMID: 5112654
Abstract
Ethidium bromide intercalates between the bases of native DNA, resulting in several biological anomalies. The effects of ethidium bromide on the mitochondria of cultured mouse L cells were studied. At a concentration of 1 µg ethidium bromide/ml it was observed that concentrations of cytochromes
a
+
a
3
and
b
decreased,
a
+
a
3
more rapidly than
b
. In contrast, the concentration of cytochromes
c
1
and
c
increased or remained the same as in control cells. Concomitant with the decrease of cytochromes
a
+
a
3
and
b
was an enlargement of the mitochondria and a reduction in the cristae. The cristae that remained were abnormally organized. After prolonged treatment with ethidium bromide a second population of small, more normally organized mitochondria was apparent. These effects of ethidium bromide could be reversed.
Metrics
Details
- Title
- EFFECTS OF ETHIDIUM BROMIDE ON THE CYTOCHROME CONTENT AND ULTRASTRUCTURE OF L CELL MITOCHONDRIA
- Creators
- Gerald Soslau - University of PennsylvaniaMargit M. K. Nass - University of Pennsylvania
- Publication Details
- The Journal of cell biology, v 51(2), pp 514-524
- Publisher
- The Rockefeller University Press
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:A1971K684700013
- Scopus ID
- 2-s2.0-0015159894
- Other Identifier
- 991020837832404721