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Journal article
EGF-R dependent regulation of keratinocyte survival
Journal of cell science, v 110 ( Pt 2)(2)
Jan 1997
PMID: 9044042
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Tissue organization and maintenance within multicellular organisms is in part dependent on the ability of cells to undergo programmed cell death or apoptosis. Conversely, disruption of cell death pathways often is associated with tumor development. At present, the molecular control of apoptosis in epithelial cells is poorly understood. Here we describe evidence linking epidermal growth factor-receptor (EGF-R) activation to survival of normal human keratinocytes in culture. Inhibition of EGF-R activation by an anti-EGF-R antagonistic monoclonal antibody (mAb 425), followed by detachment of keratinocytes from the substratum, induced extensive death with several features of apoptosis in keratinocyte cultures. Other, non-epithelial normal human cells including melanocytes and fibroblasts, did not show this effect. Similar to EGF-R blockade by mAb 425, inhibition of the EGF-R tyrosine kinase activity using tyrphostin AG1478 resulted in lack of attachment and extensive cell death upon passaging. Attachment to keratinocyte-derived ECM partially resuced mAb 425-treated keratinocytes from cell death, indicating that adhesion-dependent and EGF-R-dependent signal transduction pathways serve partially overlapping but not redundant roles in supporting keratinocyte survival.
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Details
- Title
- EGF-R dependent regulation of keratinocyte survival
- Creators
- U Rodeck - Wistar Institute, Philadelphia, PA 19104, USAM JostC KariD T ShihR M LavkerD L EwertP J Jensen
- Publication Details
- Journal of cell science, v 110 ( Pt 2)(2)
- Publisher
- Company of Biologists; England
- Grant note
- R01-AR42998 / NIAMS NIH HHS R01-AR42682 / NIAMS NIH HHS R01-EY06769 / NEI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Intensive Medical Sciences (IMS)
- Web of Science ID
- WOS:A1997WH02500002
- Scopus ID
- 2-s2.0-0031045848
- Other Identifier
- 991014878529404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology