Journal article
EPO reduces reactive gliosis and stimulates neurotrophin expression in Muller cells
Frontiers in bioscience (Elite edition), v 3(4), pp 1541-1555
01 Jun 2011
PMID: 21622158
Abstract
To characterize Müller cell-mediated neuroprotective and neurotrophic functions of the erythropoietin (EPO)/EPO receptor (EpoR) system in diabetic rat retina. A single intravitreal injection of EPO (8 mU/eye) was administered in rats 4 or 24 weeks after diabetes onset. The results showed that intravitreal EPO ameliorated the up-regulation of GFAP and vimentin in the diabetic retina evaluated by immunofluorescence and Western blotting; but up-regulated BDNF and CNTF expressions, quantified by real-time PCR and ELISA, in the 24-week diabetic rat retinas. In vitro, BDNF and CNTF expressions were stimulated by EPO through both extracellular signal-regulated kinase1/2 (ERK1/2) and Akt pathways. The neuro-regenerative function of EPO, as indicated by promotion of neurite outgrowth, was corroborated in vitro. BDNF was involved in EPO-induced neurite outgrowth of primary rat retinal neurons. Exogenous EPO exerts neuroprotective and neurotrophic functions by attenuating reactive gliosis and promoting neurotrophic factors in Muller cells in diabetic retina. Signaling pathways that are responsible for these Muller cell-mediated EPO/EpoR functions may be therapeutic targets for diabetic retinopathy.
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37 citations in Scopus
Details
- Title
- EPO reduces reactive gliosis and stimulates neurotrophin expression in Muller cells
- Creators
- Liu-Mei Hu - Peking Union Medical College HospitalYan Luo - Peking Union Medical College HospitalJingfa Zhang - Shanghai Jiao Tong UniversityXia Lei - Shanghai Jiao Tong UniversityJianfeng Shen - Shanghai Jiao Tong UniversityYalan Wu - Shanghai Jiao Tong UniversityMei Qin - Peking Union Medical College HospitalYaprak Banu Unver - Beyoglu Eye Research and Training HospitalYong Zhong - Peking Union Medical College HospitalGuo-Tong Xu - Shanghai Jiao Tong UniversityWeiye Li - Peking Union Medical College Hospital
- Publication Details
- Frontiers in bioscience (Elite edition), v 3(4), pp 1541-1555
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Ophthalmology [Historical]
- Scopus ID
- 2-s2.0-80053613637
- Other Identifier
- 991019299014904721